Voisset C, Blancher A, Perron H, Mandrand B, Mallet F, Paranhos-Baccalà G
Unité Mixte de Recherche 103 CNRS-bioMérieux, Ecole Normale Supérieure de Lyon, France.
AIDS Res Hum Retroviruses. 1999 Nov 20;15(17):1529-33. doi: 10.1089/088922299309810.
A novel human endogenous retrovirus, HERV-W, has been characterized on the basis of multiple sclerosis-associated retrovirus (MSRV) probes. We have analyzed the phylogenetic distribution of HERV-W in humans and other primate species. As HERV-W presents a C/D chimeric nature and is largely composed of deleted elements, Southern blots were performed using gag, pol, env, and LTR probes. The relative complexities observed for gag, pol, env, and LTR regions were similar in humans, apes, and Old World monkeys, the minimal number of bands observed after Southern blot analysis being 25, 50, 10, and at least 100, respectively. The HERV-W family entered the genome of catarrhines more than 25 million years ago.
一种新型人类内源性逆转录病毒HERV-W，已基于与多发性硬化症相关的逆转录病毒（MSRV）探针进行了表征。我们分析了HERV-W在人类和其他灵长类物种中的系统发育分布。由于HERV-W具有C/D嵌合性质且主要由缺失元件组成，因此使用gag、pol、env和LTR探针进行了Southern印迹分析。在人类、猿类和旧世界猴中，观察到的gag、pol、env和LTR区域的相对复杂性相似，Southern印迹分析后观察到的条带最小数量分别为25、50、10和至少100条。HERV-W家族在2500多万年前进入了狭鼻猿的基因组。
