Neilan J G, Borca M V, Lu Z, Kutish G F, Kleiboeker S B, Carrillo C, Zsak L, Rock D L
Plum Island Animal Disease Center, Agricultural Research Service, US Department of Agriculture, PO Box 848, Greenport, NY 11944-0848, USA1.
J Gen Virol. 1999 Oct;80 ( Pt 10):2693-2697. doi: 10.1099/0022-1317-80-10-2693.
An African swine fever virus (ASFV) ORF, 8CR, with similarity to the C-type lectin family of adhesion proteins has been described in the pathogenic isolate Malawi Lil-20/1. The similarity of 8CR to cellular and poxvirus genes associated with cell adhesion, cell recognition and virus infectivity suggested that 8CR may be of significance to ASFV-host cell interactions. Sequence analysis of the 8CR ORF from additional pathogenic ASFV isolates demonstrated conservation among isolates from both pig and tick sources. Northern blot analysis demonstrated 8CR mRNA transcription late in the virus replication cycle. A Malawi Lil-20/1 8CR deletion mutant (delta8CR) was constructed to analyse 8CR function further. The growth characteristics in vitro of delta8CR in porcine macrophage cell cultures were identical to those observed for parental virus. In domestic swine, delta8CR exhibited an unaltered parental Malawi Lil-20/1 disease and virulence phenotype. Thus, although well conserved among pathogenic ASFV field isolates, 8CR is non-essential for growth in porcine macrophages in vitro and for virus virulence in domestic swine.
在致病性分离株马拉维Lil-20/1中发现了一种非洲猪瘟病毒（ASFV）开放阅读框8CR，它与C型凝集素家族的粘附蛋白具有相似性。8CR与细胞粘附、细胞识别及病毒感染性相关的细胞和痘病毒基因的相似性表明，8CR可能在ASFV与宿主细胞的相互作用中具有重要意义。对其他致病性ASFV分离株的8CR开放阅读框进行序列分析，结果显示来自猪源和蜱源的分离株之间具有保守性。Northern印迹分析表明，8CR mRNA在病毒复制周期后期转录。构建了马拉维Lil-20/1 8CR缺失突变体（delta8CR）以进一步分析8CR的功能。delta8CR在猪巨噬细胞培养物中的体外生长特性与亲本病毒相同。在家猪中，delta8CR表现出与亲本马拉维Lil-20/1相同的疾病和毒力表型。因此，尽管8CR在致病性ASFV野外分离株中高度保守，但它对于体外猪巨噬细胞的生长以及家猪中的病毒毒力并非必需。
