Mutterer J D, Stussi-Garaud C, Michler P, Richards K E, Jonard G, Ziegler-Graff V
Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, 12 rue du Gé néral Zimmer, Strasbourg 67084 cedex , France1.
J Gen Virol. 1999 Oct;80 ( Pt 10):2771-2778. doi: 10.1099/0022-1317-80-10-2771.
Luteoviruses such as beet western yellows polerovirus (BWYV) are confined to and multiply within the phloem compartment of their hosts. The readthrough domain (RTD) of the minor BWYV capsid protein P74 is required for efficient virus accumulation in Nicotiana clevelandii. Experiments were carried out to determine if the low virus titres observed following agro-inoculation of whole plants with certain RTD mutants are due to a defect in virus multiplication in the nucleate cells of the phloem compartment or to inefficient virus movement to new infection sites. Immuno-localization of wild-type and an RTD-null mutant virus in thin sections of petioles and in phloem cells of leaf lamina, as well as electron microscopy observations, were all consistent with the conclusion that the RTD is not essential for efficient virus multiplication in the nucleate phloem cells but intervenes in virus movement to increase the rate at which new infection foci are established and expand.
黄症病毒属病毒，如甜菜西方黄化马铃薯卷叶病毒（BWYV），局限于宿主的韧皮部并在其中繁殖。较小的BWYV衣壳蛋白P74的通读结构域（RTD）是病毒在克利夫兰烟草中高效积累所必需的。进行了实验以确定在用某些RTD突变体对整株植物进行农杆菌接种后观察到的低病毒滴度，是由于韧皮部有核细胞中病毒繁殖缺陷，还是由于病毒向新感染部位移动效率低下。野生型和RTD缺失突变体病毒在叶柄薄片和叶片韧皮部细胞中的免疫定位，以及电子显微镜观察，均与以下结论一致：RTD对于有核韧皮部细胞中病毒的高效繁殖不是必需的，但会干预病毒移动，以提高新感染灶形成和扩展的速率。
