Department of Biology, School of Mathematics and Natural Sciences, Mongolian National University of Education, Ulaanbaatar, Mongolia.
Plant Systems Engineering Research Center, Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Arch Virol. 2021 May;166(5):1495-1499. doi: 10.1007/s00705-021-05004-2. Epub 2021 Mar 1.
The complete genome sequence of a new polerovirus found naturally infecting Artemisia princeps, artemisia virus B (ArtVB), was determined using high-throughput sequencing. The ArtVB genome comprises 6,141 nucleotides and contains six putative open reading frames (ORF0 to ORF5) with a genome structure typical of poleroviruses. A multiple sequence alignment showed that the complete ArtVB genome shares 50.98% nucleotide sequence identity with ixeridium yellow mottle virus 1 (IxYMaV-1, GenBank accession no. KT868949). ArtVB shares the highest amino acid sequence identity in P0 and P3-P5 (21.54%-51.69%) with other known poleroviruses. Phylogenetic analysis indicated that ArtVB should be considered a member of a new species within the genus Polerovirus, family Luteoviridae.
利用高通量测序技术，确定了一种新的杆状病毒——自然感染苍术的 Artemisia princeps 病毒 B（ArtVB）的完整基因组序列。ArtVB 基因组由 6141 个核苷酸组成，包含六个假定的开放阅读框（ORF0 至 ORF5），具有杆状病毒的典型基因组结构。多个序列比对显示，完整的 ArtVB 基因组与 ixeridium 黄色斑驳病毒 1（IxYMaV-1，GenBank 登录号 KT868949）的核苷酸序列同源性为 50.98%。ArtVB 在 P0 和 P3-P5 区与其他已知的杆状病毒具有最高的氨基酸序列同一性（21.54%-51.69%）。系统进化分析表明，ArtVB 应被视为 Luteoviridae 科 Polerovirus 属的一个新种成员。
