通过相互染色体涂染构建兔（穴兔）和人类的完整同源性图谱。

Complete homology maps of the rabbit (Oryctolagus cuniculus) and human by reciprocal chromosome painting.

作者信息

Korstanje R, O'Brien P C, Yang F, Rens W, Bosma A A, van Lith H A, van Zutphen L F, Ferguson-Smith M A

机构信息

Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

出版信息

Cytogenet Cell Genet. 1999;86(3-4):317-22. doi: 10.1159/000015325.

DOI:10.1159/000015325

PMID:10575232

Abstract

Fluorescence in situ hybridization (FISH) was used to construct a homology map to analyse the extent of evolutionary conservation of chromosome segments between human and rabbit (Oryctolagus cuniculus, 2n = 44). Chromosome-specific probes were established by bivariate fluorescence activated flow sorting followed by degenerate oligonucleotide-primed PCR (DOP-PCR). Painting of rabbit probes to human chromosomes and vice versa allowed a detailed analysis of the homology between these species. All rabbit chromosome paints, except for the Y paint, hybridized to human chromosomes. All human chromosome paints, except for the Y paint, hybridized to rabbit chromosomes. The results obtained revealed extensive genome conservation between the two species. Rabbit chromosomes 12, 19 and X were found to be completely homologous to human chromosomes 6, 17 and X, respectively. All other human chromosomes were homologous to two or sometimes three rabbit chromosomes. Many conserved chromosome segments found previously in other mammals (e.g. cat, pig, cattle, Indian muntjac) were also found to be conserved in rabbit chromosomes.

摘要

荧光原位杂交（FISH）被用于构建同源图谱，以分析人类与兔子（穴兔，2n = 44）之间染色体片段的进化保守程度。通过双变量荧光激活流式细胞分选，随后进行简并寡核苷酸引物PCR（DOP-PCR），建立了染色体特异性探针。用兔子探针与人染色体杂交以及反之用人类探针与兔子染色体杂交，使得能够对这些物种之间的同源性进行详细分析。除了Y染色体探针外，所有兔子染色体探针都能与人染色体杂交。除了Y染色体探针外，所有人类染色体探针都能与兔子染色体杂交。所获得的结果揭示了这两个物种之间广泛的基因组保守性。发现兔子的12号、19号染色体以及X染色体分别与人的6号、17号染色体以及X染色体完全同源。所有其他人类染色体与两条或有时三条兔子染色体同源。先前在其他哺乳动物（如猫、猪、牛、印度麂）中发现的许多保守染色体片段在兔子染色体中也被发现是保守的。