Yang F, Carter N P, Shi L, Ferguson-Smith M A
Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan.
Chromosoma. 1995 May;103(9):642-52. doi: 10.1007/BF00357691.
We have used a combination of chromosome sorting, degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), chromosome painting and digital image capturing and processing techniques for comparative chromosome analysis of members of the genus Muntiacus. Chromosome-specific "paints" from a female Indian muntjac were hybridised to the metaphase chromosomes of the Gongshan, Black, and Chinese muntjac by both single and three colour chromosome painting. Karyotypes and idiograms for the Indian, Gongshan, Black and Chinese muntjac were constructed, based on enhanced 4', 6-diamidino-2-phenylindole (DAPI) banding patterns. The hybridisation signal for each paint was assigned to specific bands or chromosomes for all of the above muntjac species. The interspecific chromosomal homology was demonstrated by the use of both enhanced DAPI banding and comparative chromosome painting. These results provide direct molecular cytogenetic evidence for the tandem fusion theory of the chromosome evolution of muntjac species.
我们运用了染色体分选、简并寡核苷酸引物聚合酶链反应（DOP-PCR）、染色体涂染以及数字图像采集与处理技术相结合的方法，对麂属成员进行比较染色体分析。来自一只雌性印度麂的染色体特异性“涂料”，通过单颜色和三颜色染色体涂染，与贡山麂、黑麂和中华麂的中期染色体进行杂交。基于增强的4',6-二脒基-2-苯基吲哚（DAPI）带型，构建了印度麂、贡山麂、黑麂和中华麂的核型和染色体模式图。为上述所有麂属物种，将每种涂料的杂交信号分配到特定的带或染色体上。通过使用增强的DAPI带型和比较染色体涂染，证明了种间染色体同源性。这些结果为麂属物种染色体进化的串联融合理论提供了直接的分子细胞遗传学证据。
