胸腺迁出细胞上L-选择素的表达定义了两条不同的组织迁移途径。
L-selectin expression on thymic emigrants defines two distinct tissue-migration pathways.
作者信息
Holder J E, Kimpton W G, Washington E A, Cahill R N
机构信息
Laboratory for Foetal and Neonatal Immunology, The University of Melborne, Parkville, Victoria, Australia.
出版信息
Immunology. 1999 Nov;98(3):422-6. doi: 10.1046/j.1365-2567.1999.00882.x.
We have studied the appearance and phenotype of recent thymic emigrants in blood, spleen and lymph nodes (LN) of neonatal lambs. Using in situ labelling of thymocytes with fluoroscein isothiocyanate (FITC), we examined the expression of the LN homing receptor L-selectin on alphabeta and gammadelta subsets of recent thymic emigrants 24 hr after labelling. There were marked differences in the proportions of CD4+, CD8+ and gammadelta T-cell receptor (TCR+) cells exported from the thymus to spleen compared to lymph nodes. Spleen was enriched in CD8+ and gammadelta TCR+ emigrants while LN were enriched in CD4+ emigrants. There were also marked differences in the expression of L-selectin by emigrants homing to spleen compared with those homing to lymph nodes. While the majority of thymic emigrants in LN expressed L-selectin, considerably fewer emigrants in spleen were L-selectin+. The presence of large numbers of CD8+ L-selectin- and gammadelta TCR+ L-selectin- thymic emigrants homing to spleen raises the possibility that unique homing receptor specificities underpin the migration of T cells to spleen as distinct from lymph nodes.
我们研究了新生羔羊血液、脾脏和淋巴结（LN）中近期胸腺迁出细胞的外观和表型。通过用异硫氰酸荧光素（FITC）对胸腺细胞进行原位标记，我们检测了标记后24小时近期胸腺迁出细胞的αβ和γδ亚群上LN归巢受体L-选择素的表达。与淋巴结相比，从胸腺输出到脾脏的CD4⁺、CD8⁺和γδ T细胞受体（TCR⁺）细胞比例存在显著差异。脾脏中富含CD8⁺和γδ TCR⁺迁出细胞，而淋巴结中富含CD4⁺迁出细胞。归巢至脾脏的迁出细胞与归巢至淋巴结的迁出细胞在L-选择素表达上也存在显著差异。虽然淋巴结中大多数胸腺迁出细胞表达L-选择素，但脾脏中表达L-选择素的迁出细胞要少得多。大量CD8⁺ L-选择素阴性和γδ TCR⁺ L-选择素阴性的胸腺迁出细胞归巢至脾脏，这增加了一种可能性，即独特的归巢受体特异性是T细胞迁移至脾脏而非淋巴结的基础。
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