新城疫病毒的一种病毒体相关酶在体外对信使核糖核酸的甲基化作用。
Methylation of messenger RNA of Newcastle disease virus in vitro by a virion-associated enzyme.
作者信息
Colonno R J, Stone H O
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2611-5. doi: 10.1073/pnas.72.7.2611.
Abstract
Purified Newcastle disease virus contains an enzyme that incorporates the methyl group from S-adenosyl-L-methionine into RNA synthesized in vitro by the virion-associated RNA polymerase (RNA nucleotidyltransferase). Incorporation of radioactivity from S-adenosyl-L-[methyl-3H]methionine was totally dependent upon RNA synthesis. The methylation reaction was completely inhibited by S-adenosyl-L-homocysteine, suggesting the transfer of only the methyl group of S-adenosyl-methionine to RNA products. Velocity sedimentation and hybridization of the in vitro product RNA indicated that both [3H]methyl and [32P]GMP labels resided in single-stranded 18S RNA molecules which were virus specific. Approximately 1 to 2 methyl groups were incorporated per RNA molecule. DEAE-cellulose chromatography of product RNA after alkaline hydrolysis suggested that the 5' terminus was the site of methylation.
摘要
纯化的新城疫病毒含有一种酶，该酶可将S-腺苷-L-甲硫氨酸的甲基基团掺入到由病毒体相关RNA聚合酶（RNA核苷酸转移酶）在体外合成的RNA中。从S-腺苷-L-[甲基-³H]甲硫氨酸掺入放射性完全依赖于RNA合成。S-腺苷-L-高半胱氨酸完全抑制甲基化反应，这表明仅将S-腺苷甲硫氨酸的甲基基团转移到RNA产物中。体外产物RNA的速度沉降和杂交表明，[³H]甲基和[³²P]GMP标记都存在于病毒特异性的单链18S RNA分子中。每个RNA分子大约掺入1至2个甲基基团。碱性水解后产物RNA的DEAE-纤维素色谱分析表明，5'末端是甲基化位点。
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