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病毒体相关甲基转移酶对新生呼肠孤病毒mRNA的体外甲基化作用。

In vitro methylation of nascent reovirus mRNA by a virion-associated methyl transferase.

作者信息

Faust M, Millward S

出版信息

Nucleic Acids Res. 1974 Dec;1(12):1739-52. doi: 10.1093/nar/1.12.1739.

DOI:10.1093/nar/1.12.1739
PMID:4449734
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343453/
Abstract

Chymotrypsin-derived cores, but not virions, catalyze the transfer of methyl groups from S-adenosyl methionine (SAM) to nascent mRNA synthesized in vitro by the core polymerase. The reaction requires Mg(++), and is dependent on the presence of all 4 ribonucleoside triphosphates (rNTPs). Methylation proceeds optimally at 51 degrees C. All ten species of mRNA become methylated during transcription and it is estimated that one methyl residue is incorporated per RNA chain. Experiments designed to determine the location of the methylated nucleotide clearly demonstrate that methylation occurs exclusively at the 5' ends of nascent mRNA.

摘要

糜蛋白酶衍生的核心颗粒而非病毒粒子，能催化甲基基团从S-腺苷甲硫氨酸（SAM）转移至核心聚合酶在体外合成的新生mRNA上。该反应需要Mg(++)，且依赖于所有4种核糖核苷三磷酸（rNTPs）的存在。甲基化在51摄氏度时进行得最为理想。所有十种mRNA在转录过程中都会发生甲基化，据估计每条RNA链会掺入一个甲基残基。旨在确定甲基化核苷酸位置的实验清楚地表明，甲基化仅发生在新生mRNA的5'端。

相似文献

1
In vitro methylation of nascent reovirus mRNA by a virion-associated methyl transferase.病毒体相关甲基转移酶对新生呼肠孤病毒mRNA的体外甲基化作用。
Nucleic Acids Res. 1974 Dec;1(12):1739-52. doi: 10.1093/nar/1.12.1739.
2
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3
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引用本文的文献

1
Reovirus progeny subviral particles synthesize uncapped mRNA.呼肠孤病毒子代亚病毒颗粒合成无帽mRNA。
J Virol. 1980 May;34(2):497-505. doi: 10.1128/JVI.34.2.497-505.1980.
2
Virion DNA-independent RNA polymerase from Saccharomyces cerevisiae.来自酿酒酵母的病毒粒子DNA非依赖性RNA聚合酶。
Nucleic Acids Res. 1980 Jun 11;8(11):2349-63. doi: 10.1093/nar/8.11.2349.
3
5'-terminal cap structure in eucaryotic messenger ribonucleic acids.真核生物信使核糖核酸中的5'-末端帽结构。
Microbiol Rev. 1980 Jun;44(2):175-205. doi: 10.1128/mr.44.2.175-205.1980.
4
mRNA capping enzymes are masked in reovirus progeny subviral particles.呼肠孤病毒子代亚病毒颗粒中的mRNA加帽酶被掩盖。
J Virol. 1980 May;34(2):490-6. doi: 10.1128/JVI.34.2.490-496.1980.
5
Reovirus guanylyltransferase is L2 gene product lambda 2.呼肠孤病毒鸟苷酸转移酶是L2基因产物λ2。
J Virol. 1986 Oct;60(1):307-11. doi: 10.1128/JVI.60.1.307-311.1986.
6
Modified 5'-termini in small nuclear RNAs of mouse myeloma cells.小鼠骨髓瘤细胞小核RNA中5'末端的修饰
Mol Biol Rep. 1975 Dec;2(4):287-94. doi: 10.1007/BF00357015.
7
Replication of double-stranded RNA in particles of Penicillium stoloniferum virus S.匐枝青霉病毒S颗粒中双链RNA的复制
Nucleic Acids Res. 1975 Oct;2(10):1889-902. doi: 10.1093/nar/2.10.1889.
8
The methylation state of poly A-containing messenger RNA from cultured hamster cells.来自培养的仓鼠细胞的含聚腺苷酸信使核糖核酸的甲基化状态。
Nucleic Acids Res. 1975 Oct;2(10):1653-68. doi: 10.1093/nar/2.10.1653.
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m7G5'ppp5'GmptcpUp at the 5' terminus of reovirus messenger RNA.呼肠孤病毒信使核糖核酸5'末端的m7G5'ppp5'GmptcpUp
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Methylation of messenger RNA of Newcastle disease virus in vitro by a virion-associated enzyme.新城疫病毒的一种病毒体相关酶在体外对信使核糖核酸的甲基化作用。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2611-5. doi: 10.1073/pnas.72.7.2611.

本文引用的文献

1
"Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA.含双链RNA的细胞质多角体病毒的病毒相关转录酶的“甲基化偶联”转录
Nucleic Acids Res. 1974 Jun;1(6):809-22. doi: 10.1093/nar/1.6.809.
2
Control of transcription of the reovirus genome.呼肠孤病毒基因组转录的调控
Nucleic Acids Res. 1974 Mar;1(3):373-85. doi: 10.1093/nar/1.3.373.
3
Reovirus: RNA polymerase activity in purified virions.呼肠孤病毒：纯化病毒颗粒中的RNA聚合酶活性
Biochem Biophys Res Commun. 1968 Dec 30;33(6):895-901. doi: 10.1016/0006-291x(68)90396-3.
4
Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳分离十种呼肠孤病毒基因组片段。
J Virol. 1968 Oct;2(10):986-91. doi: 10.1128/JVI.2.10.986-991.1968.
5
Poly(A) polymerase activity in reovirus.呼肠孤病毒中的聚腺苷酸聚合酶活性
J Virol. 1974 Jun;13(6):1338-45. doi: 10.1128/JVI.13.6.1338-1345.1974.
6
Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus.痘苗病毒中的一种酶对新合成的病毒信使核糖核酸进行甲基化修饰。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3014-8. doi: 10.1073/pnas.71.8.3014.
7
Methylated messenger RNA synthesis in vitro by purified reovirus.纯化呼肠孤病毒体外合成甲基化信使核糖核酸
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3204-7. doi: 10.1073/pnas.71.8.3204.
8
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Presence of nucleoside triphosphate phosphohydrolase activity in purified virions of reovirus.呼肠孤病毒纯化病毒粒子中核苷三磷酸磷酸水解酶活性的存在
J Virol. 1970 Sep;6(3):295-302. doi: 10.1128/JVI.6.3.295-302.1970.
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Four base-specific nucleoside 5'-triphosphatases in the subviral core of reovirus.呼肠孤病毒亚病毒核心中的四种碱基特异性核苷5'-三磷酸酶。
Nature. 1970 Mar 28;225(5239):1209-13. doi: 10.1038/2251209a0.