痘苗病毒可溶性鸟苷酸转移酶和甲基转移酶对mRNA 5'末端的修饰
Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.
作者信息
Ensinger M J, Martin S A, Paoletti E, Moss B
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2525-9. doi: 10.1073/pnas.72.7.2525.
Abstract
RNA guanylyl and methyl transferases have been solubilized from vaccinia virus cores. The guanylyl transferase specifically adds a GMP residue to the 5'-terminus of unmethylated vaccinia virus mRNA to form the structures G(5')ppp(5')Gp- and G(5')ppp(5')Ap-. Studies with [alpha-32P]GTP and [beta, gamma-32P]GTP indicated that only the alpha-phosphate is transferred. In the presence of S-adenosylmethionine, the methyl transferases convert the blocked 5'-termini to m7G(5')ppp(5')Gmp- and m7G(5')ppp(5')Amp-. Similarly, the enzymes can modify synthetic poly(A) to form the structure m7G(5')ppp(5')Amp-.
摘要
RNA鸟苷酰转移酶和甲基转移酶已从痘苗病毒核心中溶解出来。鸟苷酰转移酶特异性地将一个GMP残基添加到未甲基化的痘苗病毒mRNA的5'-末端，形成G(5')ppp(5')Gp-和G(5')ppp(5')Ap-结构。用[α-32P]GTP和[β,γ-32P]GTP进行的研究表明，只有α-磷酸被转移。在S-腺苷甲硫氨酸存在的情况下，甲基转移酶将封闭的5'-末端转化为m7G(5')ppp(5')Gmp-和m7G(5')ppp(5')Amp-。同样，这些酶可以修饰合成的聚(A)以形成m7G(5')ppp(5')Amp-结构。
相似文献
1
Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.痘苗病毒可溶性鸟苷酸转移酶和甲基转移酶对mRNA 5'末端的修饰
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2525-9. doi: 10.1073/pnas.72.7.2525.
2
Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-)methyltransferase from vaccinia virions.痘苗病毒粒子中mRNA鸟苷酸转移酶和mRNA（鸟嘌呤-7-）甲基转移酶对RNA的修饰作用。
J Biol Chem. 1975 Dec 25;250(24):9330-5.
3
Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions.从痘苗病毒颗粒中纯化mRNA鸟苷酸转移酶和mRNA（鸟嘌呤-7-）甲基转移酶。
J Biol Chem. 1975 Dec 25;250(24):9322-9.
4
Methylation of messenger RNA of Newcastle disease virus in vitro by a virion-associated enzyme.新城疫病毒的一种病毒体相关酶在体外对信使核糖核酸的甲基化作用。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2611-5. doi: 10.1073/pnas.72.7.2611.
5
6
Reaction in alphavirus mRNA capping: formation of a covalent complex of nonstructural protein nsP1 with 7-methyl-GMP.甲病毒信使核糖核酸加帽反应：非结构蛋白nsP1与7-甲基鸟苷酸形成共价复合物。
Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):507-11. doi: 10.1073/pnas.92.2.507.
7
Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus.痘苗病毒中的一种酶对新合成的病毒信使核糖核酸进行甲基化修饰。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3014-8. doi: 10.1073/pnas.71.8.3014.
8
Synthesis of mRNA guanylyltransferase and mRNA methyltransferases in cells infected with vaccinia virus.牛痘病毒感染细胞中mRNA鸟苷酸转移酶和mRNA甲基转移酶的合成
J Virol. 1977 Feb;21(2):475-83. doi: 10.1128/JVI.21.2.475-483.1977.
9
mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus. Characteristics and substrate specificity.
J Biol Chem. 1978 Nov 10;253(21):7698-702.
10
Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.对纯化痘苗病毒体外合成的病毒体相关高分子量RNA的甲基化分析。
J Virol. 1977 Jul;23(1):110-6. doi: 10.1128/JVI.23.1.110-116.1977.
引用本文的文献
1
Iron-sulfur clusters in SARS-CoV-2 exoribonuclease and methyltransferase complexes: relevance for viral genome proofreading and capping.严重急性呼吸综合征冠状病毒2（SARS-CoV-2）外切核糖核酸酶和甲基转移酶复合物中的铁硫簇：与病毒基因组校对和加帽的相关性
Nat Commun. 2025 Aug 15;16(1):7585. doi: 10.1038/s41467-025-62832-5.
2
3-Deazaguanosine inhibits SARS-CoV-2 viral replication and reduces the risk of COVID-19 pneumonia in hamster.3-脱氮鸟苷抑制严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的病毒复制，并降低仓鼠感染新型冠状病毒肺炎（COVID-19）的风险。
iScience. 2025 Mar 1;28(4):112140. doi: 10.1016/j.isci.2025.112140. eCollection 2025 Apr 18.
3
N7-methylguanosine modification in cancers: from mechanisms to therapeutic potential.癌症中的N7-甲基鸟苷修饰：从机制到治疗潜力
J Hematol Oncol. 2025 Jan 29;18(1):12. doi: 10.1186/s13045-025-01665-7.
4
Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches.通过 CAGE 和天然 RNA 测序方法探索人类猴痘病毒的转录组特征。
mSphere. 2024 Sep 25;9(9):e0035624. doi: 10.1128/msphere.00356-24. Epub 2024 Aug 27.
5
Understanding the impact of transcription byproducts and contaminants.了解转录副产物和污染物的影响。
Front Mol Biosci. 2024 Jul 10;11:1426129. doi: 10.3389/fmolb.2024.1426129. eCollection 2024.
6
Therapeutic synthetic and natural materials for immunoengineering.免疫工程用治疗性合成和天然材料
Chem Soc Rev. 2024 Feb 19;53(4):1789-1822. doi: 10.1039/d3cs00805c.
7
Chemical Modifications of mRNA Ends for Therapeutic Applications.mRNA 末端的化学修饰及其治疗应用。
Acc Chem Res. 2023 Oct 17;56(20):2814-2826. doi: 10.1021/acs.accounts.3c00442. Epub 2023 Oct 2.
8
N7-methylguanosine modification: from regulatory roles to therapeutic implications in cancer.N7-甲基鸟苷修饰：从癌症中的调控作用到治疗意义
Am J Cancer Res. 2023 May 15;13(5):1640-1655. eCollection 2023.
9
Cap analogs with a hydrophobic photocleavable tag enable facile purification of fully capped mRNA with various cap structures.带有疏水性光裂解标签的帽类似物能够方便地纯化具有各种帽结构的全长加帽 mRNA。
Nat Commun. 2023 May 11;14(1):2657. doi: 10.1038/s41467-023-38244-8.
10
The mechanism of RNA capping by SARS-CoV-2.SARS-CoV-2 的 RNA 加帽机制。
Nature. 2022 Sep;609(7928):793-800. doi: 10.1038/s41586-022-05185-z. Epub 2022 Aug 9.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores.痘苗病毒中的核糖核酸合成。I. 痘苗病毒核心中RNA的合成与释放机制。
J Mol Biol. 1970 May 28;50(1):1-18. doi: 10.1016/0022-2836(70)90100-2.
3
Transfer ribonucleic acid methylases of HeLa cells.
Arch Biochem Biophys. 1968 Mar 20;124(1):483-92. doi: 10.1016/0003-9861(68)90356-1.
4
Single-stranded deoxyribonucleic acid-specific nuclease from vaccinia virus. Purification and characterization.来自痘苗病毒的单链脱氧核糖核酸特异性核酸酶。纯化与特性鉴定。
J Biol Chem. 1974 May 25;249(10):3287-91.
5
Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus.痘苗病毒中的一种酶对新合成的病毒信使核糖核酸进行甲基化修饰。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3014-8. doi: 10.1073/pnas.71.8.3014.
6
Ribonucleoside phosphates via phosphorimidazolidate intermediates. Synthesis of pseudoadenosine 5'-triphosphate.通过磷酰咪唑酸酯中间体合成核糖核苷磷酸。假腺苷5'-三磷酸的合成。
Biochemistry. 1973 Oct 23;12(22):4458-63. doi: 10.1021/bi00746a024.
7
Polyadenylate polymerase from vaccinia virions.
Nat New Biol. 1973 Sep 12;245(141):59-63. doi: 10.1038/newbio245059a0.
8
Detection of polyadenylic acid sequences in viral and eukaryotic RNA(polu(U)-cellulose columns-poly(U) filters-fiberglass-HeLa cells-bacteriophage T4).病毒和真核生物RNA中聚腺苷酸序列的检测（聚（U）纤维素柱 - 聚（U）滤膜 - 玻璃纤维 - 海拉细胞 - 噬菌体T4）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):417-21. doi: 10.1073/pnas.69.2.417.
9
Two nucleid acid-dependent nucleoside triphosphate phosphohydrolases from vaccinia virus. Purification and characterization.来自牛痘病毒的两种核酸依赖性核苷三磷酸磷酸水解酶。纯化与特性鉴定。
J Biol Chem. 1974 May 25;249(10):3273-80.
10
Identification of the nucleoside monophosphate end-group on the product of the polynucleotide phosphorylase reaction.多核苷酸磷酸化酶反应产物中核苷单磷酸末端基团的鉴定。
Biochem Biophys Res Commun. 1966 May 25;23(4):448-52. doi: 10.1016/0006-291x(66)90748-0.
Zotero 插件