含双链RNA的细胞质多角体病毒的病毒相关转录酶的“甲基化偶联”转录
"Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA.
作者信息
Furuichi Y
出版信息
Nucleic Acids Res. 1974 Jun;1(6):809-22. doi: 10.1093/nar/1.6.809.
Abstract
S-adenosyl-L-methionine (SAM) activated the virus-associated RNA polymerase of cytoplasmic polyhedrosis virus in vitro. Synthesis of single-stranded viral RNA (mRNA) proceeded depending on the presence of SAM.A methyl residue of SAM was incorporated into an RNA molecule. A ribose moiety of adenylic acid in the 5'-terminal region of the nascent RNA was methylated in the very early stage of the transcription. The dependence of the viral transcription on the presence of SAM and the methylation of terminal nucleotide suggests that the transcription of CPV is a "methylation-coupled" reaction.
摘要
S-腺苷-L-甲硫氨酸（SAM）在体外激活了细胞质多角体病毒的病毒相关RNA聚合酶。单链病毒RNA（mRNA）的合成取决于SAM的存在。SAM的一个甲基残基被掺入到RNA分子中。新生RNA 5'-末端区域腺苷酸的核糖部分在转录的早期阶段就被甲基化。病毒转录对SAM存在的依赖性以及末端核苷酸的甲基化表明，细胞质多角体病毒的转录是一种“甲基化偶联”反应。
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