Braverman Y, Chizov-Ginzburg A, Saran A, Winkler M
Kimron Veterinary Institute, Beit Dagan, Israel.
Rev Sci Tech. 1999 Dec;18(3):681-90. doi: 10.20506/rst.18.3.1187.
A study was conducted to assess the role of houseflies, Musca domestica L. in harbouring Corynebacterium pseudotuberculosis in dairy farms in Israel. The bacterium was isolated in June 1993 from 40 wild houseflies which had fed on a lesion on a cow, and from 28 laboratory flies fed on contaminated milk from a cow infected with mastitis. The bacterium was recovered from the body surface of 10 flies (of a total of 160) 10 min after being dipped entirely in a bacterial broth. The bacterium was recovered from the body surface of 10 flies (of a total of 40) 5 min after being fed on contaminated milk. When 110 flies were fed on contaminated sugar cubes, the bacterium was recovered externally from 70 flies 5 min later, and from an additional 20 flies 10 min after feeding. Of 110 flies, 80 excreted bacteria in saliva from 5 min to 3 h after feeding on contaminated milk. Bacteria were isolated from the intestine of 40 of 60 flies between 1 h and 4 h after feeding on contaminated milk. Bacteria were found in the faeces of 30 of 60 flies, between 1 h and 4 h after feeding on contaminated milk. In the light of these findings, and given the fact that this species of fly has a predilection to feed on milk residues of cow teats, the authors concluded that the housefly plays an important role in harbouring and disseminating C. pseudotuberculosis in dairy herds in Israel. In contrast, stable flies (Stomoxys calcitrans L.) are not important in the habouring and dissemination of the bacteria, since bacteria were not recovered 5, 10, 15, 30 min, 2 h or 24 h after membrane feeding on a mixture of bacterial broth and blood.
开展了一项研究，以评估家蝇（Musca domestica L.）在以色列奶牛场中携带伪结核棒状杆菌方面所起的作用。1993年6月，从40只曾吸食奶牛伤口处物质的野生家蝇以及28只取食患有乳腺炎奶牛的受污染牛奶的实验室家蝇中分离出了该细菌。将160只家蝇完全浸入细菌培养液中10分钟后，从其中10只（共160只）家蝇的体表分离出了该细菌。让40只家蝇取食受污染牛奶5分钟后，从其中10只（共40只）家蝇的体表分离出了该细菌。当110只家蝇取食受污染的糖块后，5分钟后从70只家蝇的体外分离出了该细菌，取食10分钟后又从另外20只家蝇中分离出了该细菌。在110只家蝇中，80只在取食受污染牛奶后5分钟到3小时之间通过唾液排出了细菌。在60只家蝇中，40只在取食受污染牛奶后1小时到4小时之间，其肠道中分离出了细菌。在60只家蝇中，30只在取食受污染牛奶后1小时到4小时之间，其粪便中发现了细菌。鉴于这些发现，并且考虑到这种家蝇偏好取食奶牛乳头的乳汁残留这一事实，作者得出结论，家蝇在以色列奶牛群中携带和传播伪结核棒状杆菌方面起着重要作用。相比之下，厩螫蝇（Stomoxys calcitrans L.）在携带和传播该细菌方面并不重要，因为在用细菌培养液和血液的混合物进行膜饲后5分钟、10分钟、15分钟、30分钟、2小时或24小时均未分离出细菌。
