Esclatine A, Lemullois M, Servin A L, Quero A M, Geniteau-Legendre M
Institut National de la Santé et de la Recherche Médicale, Unité 510, Pathogènes et Fonctions des Cellules Epithéliales Polarisées, Faculté de Pharmacie, Université Paris XI, 92296 Châtenay-Malabry Cedex, France.
J Virol. 2000 Jan;74(1):513-7. doi: 10.1128/jvi.74.1.513-517.2000.
Human cytomegalovirus (CMV) causes severe disease in immunosuppressed patients and notably infects the gastrointestinal tract. To understand the interaction of CMV with intestinal epithelial cells, which are highly susceptible to CMV infection in vivo, we used the intestinal epithelial cell line Caco-2 and demonstrated that CMV enters predominantly through the basolateral surface of polarized Caco-2 cells. As shown by expression of all three classes of CMV proteins and by visualization of nucleocapsids by transmission electron microscopy, both poorly and fully differentiated Caco-2 cells were permissive to CMV replication. However, infection failed to produce infectious particles in Caco-2 cells, irrespective of the state of differentiation.
人巨细胞病毒（CMV）在免疫抑制患者中引发严重疾病，尤其会感染胃肠道。为了解CMV与体内极易受CMV感染的肠上皮细胞之间的相互作用，我们使用了肠上皮细胞系Caco-2，并证明CMV主要通过极化的Caco-2细胞的基底外侧表面进入。如三类CMV蛋白的表达以及通过透射电子显微镜对核衣壳的观察所示，未充分分化和完全分化的Caco-2细胞均允许CMV复制。然而，无论分化状态如何，感染均未能在Caco-2细胞中产生感染性颗粒。
