OrganoVIR Lab, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, 1100 AZ Amsterdam, The Netherlands.
Department of Pediatrics Infectious Diseases, Emma Children's Hospital, Amsterdam UMC, Academic Medical Center, University of Amsterdam, 1100 AZ Amsterdam, The Netherlands.
Viruses. 2020 Nov 11;12(11):1288. doi: 10.3390/v12111288.
The knowledge about enteric viral infection has vastly increased over the last eight years due to the development of intestinal organoids and enteroids that suppose a step forward from conventional studies using cell lines. Intestinal organoids and enteroids are three-dimensional (3D) models that closely mimic intestinal cellular heterogeneity and organization. The barrier function within these models has been adapted to facilitate viral studies. In this review, several adaptations (such as organoid-derived two-dimensional (2D) monolayers) and original intestinal 3D models are discussed. The specific advantages and applications, as well as improvements of each model are analyzed and an insight into the possible path for the field is given.
由于肠类器官和肠原代细胞的发展，人们对肠病毒感染的认识在过去八年中大大提高，这比使用细胞系的传统研究前进了一步。肠类器官和肠原代细胞是三维（3D）模型，它们非常接近地模拟了肠道细胞的异质性和组织。这些模型中的屏障功能已经过调整，以方便进行病毒研究。在这篇综述中，讨论了几种适应（例如类器官衍生的二维（2D）单层）和原始的肠道 3D 模型。分析了每种模型的具体优势和应用以及改进，并对该领域的可能发展方向进行了探讨。
