Sinzger C, Plachter B, Stenglein S, Jahn G
Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Germany.
J Infect Dis. 1993 Jun;167(6):1427-32. doi: 10.1093/infdis/167.6.1427.
To analyze the expression of different human cytomegalovirus (HCMV) antigens in gastric biopsy samples from a renal transplant recipient suffering from HCMV gastritis, monoclonal antibodies (MAbs) were used in immunohistochemical analyses. In samples obtained before the start of specific therapy with ganciclovir, MAbs against immediate-early (E13), early (CCH2), and late antigen (XP1) reacted with cells in the smooth muscle, stromal, and epithelial layers. MAb E13 stained morphologically altered and unaltered cells; MAbs CCH2 and XP1 predominantly stained cytomegalic cells. After therapy with ganciclovir was begun, the three viral antigens were almost exclusively found in the epithelial cell layer, whereas viral antigen appeared to be cleared from smooth muscle and stromal cells. All cells stained by MAb E13 in these samples showed characteristic morphologic alterations, in contrast to cells in earlier samples. The changes in the pattern of viral antigen expression may reflect the effects of antiviral therapy.
为分析来自一名患有巨细胞病毒（HCMV）胃炎的肾移植受者的胃活检样本中不同人巨细胞病毒抗原的表达情况，在免疫组织化学分析中使用了单克隆抗体（MAb）。在开始使用更昔洛韦进行特异性治疗之前获取的样本中，针对即刻早期（E13）、早期（CCH2）和晚期抗原（XP1）的单克隆抗体与平滑肌、基质和上皮层中的细胞发生反应。单克隆抗体E13对形态改变和未改变的细胞进行染色；单克隆抗体CCH2和XP1主要对巨细胞进行染色。开始使用更昔洛韦治疗后，这三种病毒抗原几乎仅在上皮层细胞中发现，而病毒抗原似乎已从平滑肌和基质细胞中清除。与早期样本中的细胞相比，这些样本中被单克隆抗体E13染色的所有细胞均显示出特征性形态改变。病毒抗原表达模式的变化可能反映了抗病毒治疗的效果。
