Burdon T, Chambers I, Stracey C, Niwa H, Smith A
Centre for Genome Research, University of Edinburgh, UK.
Cells Tissues Organs. 1999;165(3-4):131-43. doi: 10.1159/000016693.
An ability to propagate pluripotent embryonic cells in culture is the foundation both for defined germline modification in experimental rodents and for future possibilities for broad-based cellular transplantation therapies in humans. Yet, the molecular basis of the self-renewing pluripotent phenotype remains ill-defined. The relationship between factors that influence embryonic stem cell propagation in vitro and mechanisms of stem cell regulation operative in the embryo is also uncertain. In this article we discuss the role of intracellular signalling pathways in the maintenance of pluripotency and induction of differentiation in embryonic stem cell cultures and the mammalian embryo.
在培养中增殖多能胚胎细胞的能力，既是在实验啮齿动物中进行明确的种系修饰的基础，也是未来人类广泛开展细胞移植治疗的可能性基础。然而，自我更新的多能表型的分子基础仍然不清楚。影响胚胎干细胞体外增殖的因素与胚胎中起作用的干细胞调节机制之间的关系也不确定。在本文中，我们讨论细胞内信号通路在胚胎干细胞培养物和哺乳动物胚胎中多能性维持及分化诱导中的作用。
