Hunt P S, Trotter S
Aust N Z J Surg. 1975 May;45(2):214-9. doi: 10.1111/j.1445-2197.1975.tb05764.x.
The action in culture of peripheral blood lymphocytes on autologous large-intestinal epithelial cells was studied in 13 patients with severe mucosal ulcerative colitis. Two different methods were used to measure lymphocyte activity. These showed that autologous-lymphocyte-induced release of isotopic label and detachment in monolayer culture of large-intestinal epithelial cells was increased in acute ulcerative colitis when compared with findings in the same studies in six normal subjects. Subsequently in four of the six patients who responded to cortisone it was shown that lymphocyte activity against epithelial cells returned to the normal range. Further control studies showed little lymphocyte activity against autologous skin and ileum, suggesting that autologous-lymphocyte-induced damage of large-intestinal epithelial cells is a tissue-specific reaction in patients with acute ulcerative colitis. The absence of reactivity in other colonic inflammatory diseases also suggested that such increased in vitro lymphocyte activity is disease-specific for ulcerative colitis.
对13例重度黏膜溃疡性结肠炎患者外周血淋巴细胞在体外对自体大肠上皮细胞的作用进行了研究。采用两种不同方法检测淋巴细胞活性。结果显示，与6名正常受试者的相同研究结果相比，急性溃疡性结肠炎患者的自体淋巴细胞诱导的大肠上皮细胞单层培养中同位素标记释放及细胞脱离增加。随后，在6例对皮质醇有反应的患者中的4例显示，淋巴细胞对上皮细胞的活性恢复到正常范围。进一步的对照研究显示，淋巴细胞对自体皮肤和回肠几乎没有活性，提示自体淋巴细胞诱导的大肠上皮细胞损伤是急性溃疡性结肠炎患者的一种组织特异性反应。其他结肠炎症性疾病中无反应性也提示，这种体外淋巴细胞活性增加是溃疡性结肠炎所特有的。
