Hunt P S, Trotter S
Aust N Z J Surg. 1976 Feb;46(1):88-91. doi: 10.1111/j.1445-2197.1976.tb03205.x.
Large-intestinal epithelial cells were obtained from operation specimens and by biopsy at sigmoidoscopy from 16 patients with acute severe mucosal ulcerative colitis and II control subjects with normal large-bowel mucosa. The ability of lymphocytes to release 51Cr label from large-intestinal epithelial cells was quantitatively assessed as percentage lymphocyte activity. The mean +/- standard deviation of percentage lymphocyte activity in 16 patients with acute ulcerative colitis on admission to hospital (88 +/- 8) was significantly greater than that in II normal subjects (16 +/- 8) (P is less than 0.01). In each study, samples of autologous serum were added to two reactions between lymphocytes and autologous large-intestinal epithelial cells. The results in the seven patients who came to urgent colectomy differed significantly from those in the nine who responded to corticosteroids. The mean +/- S.D. of percentage serological inhibition of lymphocyte activity in the group coming to colectomy was 44 +/- 16, and in those responding to steroids 12 +/- 5. When the serum of these patients was studied for immunofluorescent autoantibodies to normal human colonic mucosa, none of the seven patients treated by colectomy had detectable autoantibodies, in contrast with three of the nine patients with a more favourable short-term prognosis, in that they responded to steroid treatment.
从16例急性重症黏膜溃疡性结肠炎患者的手术标本以及通过乙状结肠镜活检获取大肠上皮细胞，并从11例大肠黏膜正常的对照受试者获取细胞。淋巴细胞从大肠上皮细胞释放51Cr标记物的能力以淋巴细胞活性百分比进行定量评估。16例急性溃疡性结肠炎患者入院时淋巴细胞活性百分比的平均值±标准差为88±8，显著高于11例正常受试者（16±8）（P<0.01）。在每项研究中，将自体血清样本添加到淋巴细胞与自体大肠上皮细胞之间的两个反应中。7例行急诊结肠切除术的患者的结果与9例对皮质类固醇有反应的患者的结果有显著差异。行结肠切除术组淋巴细胞活性血清学抑制百分比的平均值±标准差为44±16，而对类固醇有反应的患者为12±5。当研究这些患者血清中针对正常人结肠黏膜的免疫荧光自身抗体时，7例行结肠切除术的患者均未检测到自身抗体，相比之下，9例短期预后较好（即对类固醇治疗有反应）的患者中有3例检测到自身抗体。
