Hunt P S, Trotter S
Gut. 1973 Nov;14(11):875-9. doi: 10.1136/gut.14.11.875.
The blastic transformation in vitro of peripheral blood lymphocytes was measured by the 72-hour uptake of tritiated thymidine ((3)H-6-thymidine) in 23 patients with mucosal ulcerative colitis, three patients with acute Crohn's colitis with rectal involvement, and seven normal subjects. The 23 patients with ulcerative colitis were subdivided into three groups, graded according to severity into seven with acute, severe, nine with active, chronic, and seven with quiescent disease. In the control cultures of lymphocytes without any added potential stimulant the uptake of (3)H-6-thymidine in the clinical subgroup of seven patients with acute, severe ulcerative colitis was significantly greater than in seven normal subjects (p<0.01). This contrasted with a reduced uptake of (3)H-6-thymidine by lymphocytes from seven patients with acute severe colitis when compared with seven normal subjects after stimulation with phytohaemagglutinin-P (PHA-P) (p<0.01). In further duplicate cultures of lymphocytes specifically stimulated by an equal number of viable autologous rectal epithelial cells, the uptake of (3)H-6-thymidine was significantly greater in seven patients with acute severe colitis when compared with seven normal subjects (p<0.01). The results in three patients with acute Crohn's colitis with rectal involvement showed no such evidence of lymphocyte sensitivity to autologous rectal epithelial cells and their uptake of (3)H-6-thymidine lay within the normal range.Evidence that the degree of lymphoblastic transformation was related to the clinical severity of ulcerative colitis was provided by the results obtained in the unstimulated and epithelial cell stimulant cultures. The uptake of (3)H-6-thymidine was directly related to the clinical severity of ulcerative colitis in the three subgroups studied. In addition, four of the seven patients with acute severe colitis were studied later in clinical remission. They were then found to have a significantly reduced uptake of (3)H-6-thymidine in response to autologous rectal epithelial cells (p < 0.01).
通过测量23例黏膜溃疡性结肠炎患者、3例累及直肠的急性克罗恩结肠炎患者及7名正常受试者外周血淋巴细胞72小时对氚标记胸腺嘧啶核苷（³H-6-胸腺嘧啶核苷）的摄取量，来检测外周血淋巴细胞的体外原始细胞转化情况。23例溃疡性结肠炎患者被分为三组，根据病情严重程度分级，其中7例为急性重症，9例为活动期慢性，7例为静止期。在未添加任何潜在刺激物的淋巴细胞对照培养物中，7例急性重症溃疡性结肠炎临床亚组患者的³H-6-胸腺嘧啶核苷摄取量显著高于7名正常受试者（p<0.01）。相比之下，7例急性重症结肠炎患者的淋巴细胞在用植物血凝素-P（PHA-P）刺激后，其³H-6-胸腺嘧啶核苷摄取量低于7名正常受试者（p<0.01）。在由等量活的自体直肠上皮细胞特异性刺激的淋巴细胞重复培养物中，7例急性重症结肠炎患者的³H-6-胸腺嘧啶核苷摄取量显著高于7名正常受试者（p<0.01）。3例累及直肠的急性克罗恩结肠炎患者的结果未显示淋巴细胞对自体直肠上皮细胞敏感的证据，其³H-6-胸腺嘧啶核苷摄取量在正常范围内。未刺激和上皮细胞刺激培养物所获结果表明，原始细胞转化程度与溃疡性结肠炎的临床严重程度相关。在所研究的三个亚组中，³H-6-胸腺嘧啶核苷摄取量与溃疡性结肠炎的临床严重程度直接相关。此外，7例急性重症结肠炎患者中有4例随后进入临床缓解期。此时发现，他们对自体直肠上皮细胞刺激的³H-6-胸腺嘧啶核苷摄取量显著降低（p<0.01）。
