Fujimori F, Takahashi K, Uchida C, Uchida T
GeneSurf, Advanced Research Institute for Science and Engineering, Waseda University, Tokyo, Japan.
Biochem Biophys Res Commun. 1999 Nov 30;265(3):658-63. doi: 10.1006/bbrc.1999.1736.
The peptidyl prolil cis/trans isomerase Ess1/Pin1 is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. To investigate the function of Pin1 in mammalian cells, we created mice lacking Pin1. These mice underwent normal development. Although the embryonic Pin1-/- fibroblasts grew normally, they proved significantly deficient in their ability to restart proliferation in response to serum stimulation after G(0) arrest. These results suggest that Pin1 is required for cell cycle progression from G(0) arrest as well as mitosis progression in normal mammalian cells.
肽基脯氨酰顺/反异构酶Ess1/Pin1对酵母细胞的有丝分裂进程至关重要，据推测在哺乳动物细胞中也发挥相同作用。为了研究Pin1在哺乳动物细胞中的功能，我们培育了缺乏Pin1的小鼠。这些小鼠发育正常。尽管胚胎期的Pin1-/-成纤维细胞生长正常，但在G(0)期停滞之后，它们对血清刺激做出反应而重新开始增殖的能力明显不足。这些结果表明，在正常哺乳动物细胞中，从G(0)期停滞进入细胞周期进程以及有丝分裂进程都需要Pin1。
