抗原结合细胞对抗抗原-酶复合物的结合作为免疫诊断的一种方法。
Binding of antigen-enzyme complex by antigen-binding cells as an approach for immunogdiagnosis.
作者信息
Modabber F Z, Bahr G M
出版信息
Infect Immun. 1979 Jan;23(1):49-53. doi: 10.1128/iai.23.1.49-53.1979.
Abstract
An immunoenzyme method capable of detecting specific antigen-binding cells is developed. The method is based on the chemical coupling of the antigen to beta-galactosidase and binding of the conjugate to the corresponding antigen-binding cells. Human hydatid fluid was used as an antigen model, and cells derived from rabbits immunized with human hydatid fluid showed specific binding to human hydatid fluid-beta-galactosidase conjugate. The method is specific and reproducible and could be used as an immunodiagnostic test for various diseases in which little or no antibody is produced.
摘要
开发了一种能够检测特异性抗原结合细胞的免疫酶方法。该方法基于抗原与β-半乳糖苷酶的化学偶联以及偶联物与相应抗原结合细胞的结合。用人包虫液作为抗原模型，用人包虫液免疫的兔来源细胞与人包虫液-β-半乳糖苷酶偶联物表现出特异性结合。该方法具有特异性和可重复性，可作为对产生很少或不产生抗体的各种疾病的免疫诊断试验。
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本文引用的文献
1
Contributions of studies on the beta-galactosidase of Escherichia coli to our understanding of enzyme synthesis.大肠杆菌β-半乳糖苷酶研究对我们理解酶合成的贡献。
Bacteriol Rev. 1957 Sep;21(3):140-68. doi: 10.1128/br.21.3.140-168.1957.
2
Antigen binding to cells: determination by enzymic fluorogenic group hydrolysis.抗原与细胞的结合：通过酶促荧光基团水解进行测定
Science. 1968 Feb 23;159(3817):884-5. doi: 10.1126/science.159.3817.884.
3
Antigen binding and the immune response. I. The early primary response to a protein antigen.抗原结合与免疫反应。I. 对蛋白质抗原的早期初次反应。
J Immunol. 1970 Aug;105(2):355-61.
4
Antigen binding and the immune response. II. The large number of antigen-binding cells in primed animals.抗原结合与免疫反应。II. 致敏动物中大量的抗原结合细胞。
Proc Soc Exp Biol Med. 1970 Nov;135(2):400-5. doi: 10.3181/00379727-135-35061.
5
Antigen-binding cells in normal mouse thymus.正常小鼠胸腺中的抗原结合细胞。
Science. 1970 Dec 4;170(3962):1102-4. doi: 10.1126/science.170.3962.1102.
6
A simple method for detecting antibodies to rubella.一种检测风疹抗体的简单方法。
Br J Exp Pathol. 1975 Aug;56(4):338-9.
7
Enzyme immunoassays in diagnostic medicine. Theory and practice.诊断医学中的酶免疫测定。理论与实践。
Bull World Health Organ. 1976;53(1):55-65.
8
Characterization of T and B antigen-binding cells for beta-galactosidase. II. T antigen-binding cells.β-半乳糖苷酶T和B抗原结合细胞的特性。II. T抗原结合细胞。
J Immunol. 1976 Apr;116(4):923-8.
9
Enzyme immunoassays for parasitic diseases.用于寄生虫病的酶免疫测定法。
Trans R Soc Trop Med Hyg. 1976;70(2):98-106. doi: 10.1016/0035-9203(76)90163-2.
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