Merzouk H, Madani S, Chabane Sari D, Prost J, Bouchenak M, Belleville J
Laboratoire de Physiologie Animale, Université de Tlemcen, Algerie.
Clin Sci (Lond). 2000 Jan;98(1):21-30. doi: 10.1042/cs0980021.
The aim of this investigation was to determine the time course of changes in serum glucose, insulin and lipid levels, as well as lipid and protein content and lipolytic activities in insulin target organs (liver, adipose tissue and muscle), in macrosomic offspring of streptozotocin-induced mildly hyperglycaemic rats. Food intake and nutritional efficiency were also evaluated. Mild hyperglycaemia in pregnant rats was induced by intraperitoneal injection of streptozotocin (40 mg/kg body weight) on day 5 of gestation. Control pregnant rats were injected with citrate buffer. At birth, macrosomic pups (birth weight >1.7 S.D. greater than the mean value for the control pups) had higher serum insulin, glucose and lipid levels than control pups. These macrosomic rats maintained accelerated postnatal growth combined with high adipose tissue weight up to 12 weeks of age. These rats were not hyperphagic; however, they had higher food efficiency and fat storage capacity with higher adipocyte lipoprotein lipase activity, which contributed to persisting obesity. Hepatic lipase activity was increased in macrosomic rats at all ages. Moreover, macrosomia was associated with metabolic disturbances that varied according to age and sex. After 1 month, several alterations observed at birth had disappeared. Serum glucose, insulin and lipid levels in male and female macrosomic rats became similar to those of their respective controls. At 2 months of age, hepatic and serum triacylglycerol levels were higher in macrosomic females than in controls. By 3 months, macrosomic rats (both males and females) had developed insulin resistance with hyperinsulinaemia, hyperglycaemia, and higher serum and hepatic lipids. In conclusion, macrosomia was associated with alterations in glucose and lipid metabolism through to adulthood. It should be considered as an important potential risk factor for obesity and its metabolic complications.
本研究的目的是确定链脲佐菌素诱导的轻度高血糖大鼠的巨大儿后代血清葡萄糖、胰岛素和脂质水平的变化时间进程，以及胰岛素靶器官（肝脏、脂肪组织和肌肉）中的脂质和蛋白质含量及脂解活性。还评估了食物摄入量和营养效率。妊娠第5天，通过腹腔注射链脲佐菌素（40 mg/kg体重）诱导孕鼠轻度高血糖。对照孕鼠注射柠檬酸盐缓冲液。出生时，巨大儿幼崽（出生体重比对照幼崽的平均值高>1.7个标准差）的血清胰岛素、葡萄糖和脂质水平高于对照幼崽。这些巨大儿大鼠在出生后12周龄前维持加速生长，并伴有较高的脂肪组织重量。这些大鼠并非食欲亢进；然而，它们具有更高的食物效率和脂肪储存能力，脂肪细胞脂蛋白脂肪酶活性更高，这导致了持续性肥胖。各年龄段的巨大儿大鼠肝脏脂肪酶活性均增加。此外，巨大儿与随年龄和性别而异的代谢紊乱有关。1个月后，出生时观察到的几种改变消失了。雄性和雌性巨大儿大鼠的血清葡萄糖、胰岛素和脂质水平与各自的对照相似。在2月龄时，巨大儿雌性大鼠的肝脏和血清三酰甘油水平高于对照。到3个月时，巨大儿大鼠（雄性和雌性）均出现胰岛素抵抗，伴有高胰岛素血症、高血糖以及更高的血清和肝脏脂质。总之，巨大儿与直至成年期的葡萄糖和脂质代谢改变有关。它应被视为肥胖及其代谢并发症的一个重要潜在危险因素。
