Jeffrey F M, Reshetov A, Storey C J, Carvalho R A, Sherry A D, Malloy C R
Department of Radiology, The Mary Nell and Ralph B. Rogers Magnetic Resonance Center, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9085, USA.
Am J Physiol. 1999 Dec;277(6):E1111-21. doi: 10.1152/ajpendo.1999.277.6.E1111.
A kinetic model of the citric acid cycle for calculating oxygen consumption from (13)C nuclear magnetic resonance (NMR) multiplet data has been developed. Measured oxygen consumption (MVO(2)) was compared with MVO(2) predicted by the model with (13)C NMR data obtained from rat hearts perfused with glucose and either [2-(13)C]acetate or [3-(13)C]pyruvate. The accuracy of MVO(2) measured from three subsets of NMR data was compared: glutamate C-4 and C-3 resonance areas; the doublet C4D34 (expressed as a fraction of C-4 area); and C-4 and C-3 areas plus several multiplets of C-2, C-3, and C-4. MVO(2) determined by set 2 (C4D34 only) gave the same degree of accuracy as set 3 (complete data); both were superior to set 1 (C-4 and C-3 areas). Analysis of the latter suffers from the correlation between citric acid cycle flux and exchange between alpha-ketoglutarate and glutamate, resulting in greater error in estimating MVO(2). Analysis of C4D34 is less influenced by correlation between parameters, and this single measurement provides the best opportunity for a noninvasive measurement of oxygen consumption.
已建立一种用于根据碳-13核磁共振（NMR）多重峰数据计算耗氧量的柠檬酸循环动力学模型。将测得的耗氧量（MVO₂）与该模型根据从灌注葡萄糖以及[2-(¹³)C]乙酸盐或[3-(¹³)C]丙酮酸盐的大鼠心脏获得的碳-13 NMR数据预测的MVO₂进行比较。比较了从NMR数据的三个子集测得的MVO₂的准确性：谷氨酸C-4和C-3共振面积；双峰C4D34（表示为C-4面积的分数）；以及C-4和C-3面积加上C-2、C-3和C-4的几个多重峰。由第2组（仅C4D34）确定的MVO₂与第3组（完整数据）具有相同的准确度；两者均优于第1组（C-4和C-3面积）。对后者的分析受到柠檬酸循环通量与α-酮戊二酸和谷氨酸之间交换的相关性的影响，导致在估计MVO₂时产生更大的误差。对C4D34的分析受参数之间相关性的影响较小，并且这种单一测量为无创测量耗氧量提供了最佳机会。
