Underhill D M, Bassetti M, Rudensky A, Aderem A
Department of Immunology, University of Washington, Seattle, Washington 98195, USA.
J Exp Med. 1999 Dec 20;190(12):1909-14. doi: 10.1084/jem.190.12.1909.
We have established a method for real-time video analysis of the interaction of antigen-presenting cells (APCs) with T cells. Green fluorescent protein expression controlled by a nuclear factor of activated T cells (NFAT)-responsive promoter permits the visualization of productive antigen presentation in single T cells. The readout is rapid (within 2 h) and semiquantitative and allows analysis by video microscopy and flow cytometry. Using this approach, we demonstrate that macrophages have the capacity to simultaneously activate multiple T cells. In addition, the interaction of T cells with macrophages is extraordinarily dynamic: after initial stable contact, the T cells migrate continuously on the surface of the macrophage and from APC to APC during productive antigen presentation. Thus, T cells sum up signals from multiple interactions with macrophages during stimulation.
我们已经建立了一种用于实时视频分析抗原呈递细胞（APC）与T细胞相互作用的方法。由活化T细胞核因子（NFAT）响应启动子控制的绿色荧光蛋白表达允许在单个T细胞中可视化有效的抗原呈递。读出过程快速（2小时内）且半定量，并允许通过视频显微镜和流式细胞术进行分析。使用这种方法，我们证明巨噬细胞有能力同时激活多个T细胞。此外，T细胞与巨噬细胞的相互作用非常动态：在最初的稳定接触后，T细胞在巨噬细胞表面持续迁移，并在有效的抗原呈递过程中从一个APC迁移到另一个APC。因此，T细胞在刺激过程中汇总来自与巨噬细胞多次相互作用的信号。
