Brosterhus H, Brings S, Leyendeckers H, Manz R A, Miltenyi S, Radbruch A, Assenmacher M, Schmitz J
Miltenyi Biotec GmbH Bergisch Gladbach, Germany.
Eur J Immunol. 1999 Dec;29(12):4053-9. doi: 10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C.
Following appropriate antigen-specific stimulation, CD4(+) and CD8(+) T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell surface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4(+) and CD8(+) T lymphocytes. The power of this technique is demonstrated here for HLA-A0201-restricted influenza matrix protein peptide 58-66-specific CD8(+) cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.
在适当的抗原特异性刺激后，CD4(+)和CD8(+) T淋巴细胞会迅速表达细胞因子。基于这种刺激诱导的细胞因子分泌，并利用细胞表面亲和基质技术，我们开发了一种新方法，该方法能够特异性、快速且高效地检测、分离和鉴定活的抗原特异性CD4(+)和CD8(+) T淋巴细胞。本文展示了该技术对于HLA-A0201限制性流感基质蛋白肽58 - 66特异性CD8(+)细胞毒性T淋巴细胞、甲型流感病毒和重组破伤风毒素C片段特异性Th1细胞以及破伤风类毒素特异性Th2细胞的作用。
