Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden; and.
Am J Bot. 1999 Dec;86(12):1742-53.
The sequence divergence of chloroplast rbcL, matK, trnV intron, and rpl20-rps18 spacer regions was analyzed among 32 Pinus species and representatives of six other genera in Pinaceae. The total aligned sequence length is 3570 bp. Of the four sequences examined, matK evolved much faster than rbcL in Pinus and in other Pinaceae genera. The two noncoding regions did not show more divergence than the two coding regions, especially within each Pinus subgenus. Phylogenetic analyses based on these four sequences gave consistent results and strongly supported the monophyly hypothesis for the genus Pinus and its two recognized subgenera. Pinus krempfii, the two-flat-needle pine endemic to Vietnam, was placed in subgen. Strobus and showed closer affinity to subsect. Gerardianae. The ancient character of sect. Parrya is further confirmed. However, monophyly of the sect. Parrya is not supported by our data. Among the Eurasian pines of subgen. Pinus, Mediterranean pines formed one clade and the Asian members of subsect. Sylvestres formed another. The Himalayan P. roxburghii showed considerable divergence from all the other hard pines from both regions. Pinus merkusii was distinctly separated from all the Asian members of subsect. Sylvestres. The implications of our results for Pinus classification are discussed.
对来自 32 个松属物种和松科其他 6 属代表的叶绿体 rbcL、matK、trnV 内含子和 rpl20-rps18 间隔区的序列分歧进行了分析。总对齐序列长度为 3570bp。在所研究的四个序列中，matK 在松属和其他松科属中的进化速度明显快于 rbcL。两个非编码区的分化程度并不高于两个编码区，特别是在每个松亚属内。基于这四个序列的系统发育分析给出了一致的结果，并强烈支持松属及其两个公认的亚属的单系假说。越南特有的二扁平针叶松 Pinus krempfii 被置于 strobus 亚属中，与 gerardianae 亚组关系更为密切。进一步证实了 Parrya 组的古老特征。然而，我们的数据不支持 Parrya 组的单系性。在松属的欧亚松中，地中海松形成一个分支，亚洲的 Sylvestres 亚组形成另一个分支。喜马拉雅的 P. roxburghii 与来自两个地区的所有其他硬松都有很大的分歧。松属 merkusii 与所有亚洲 Sylvestres 亚组的成员明显分开。讨论了我们的结果对松属分类的影响。
