用于分析近缘物种的引物设计：非编码线粒体DNA和叶绿体DNA序列的应用

Primer Design for the Analysis of Closely Related Species: Application of Noncoding mtDNA and cpDNA Sequences.

作者信息

Skuza Lidia

机构信息

Institute of Biology, University of Szczecin, Szczecin, Poland.

The Centre for Molecular Biology and Biotechnology, University of Szczecin, Szczecin, Poland.

出版信息

Methods Mol Biol. 2022;2392:83-91. doi: 10.1007/978-1-0716-1799-1_6.

DOI:10.1007/978-1-0716-1799-1_6

PMID:34773616

Abstract

Noncoding regions of the chloroplast (cpDNA) and mitochondrial (mtDNA) genomes are commonly used in plant phylogenetic and population studies. Consensus primers, which are homologous to most coding regions, but amplify variable noncoding regions, are very useful for this purpose. However, high genetic diversity of plants poses a problem in developing molecular methods that require conserved DNA sequences between species.This chapter describes the protocol for designing PCR primers suitable for analysis of closely related plant species. As an example, we used PCR primer design for cpDNA noncoding regions of the rye (Secale).

摘要

叶绿体（cpDNA）和线粒体（mtDNA）基因组的非编码区域常用于植物系统发育和种群研究。共有引物与大多数编码区域同源，但能扩增可变的非编码区域，为此目的非常有用。然而，植物的高遗传多样性给开发需要物种间保守DNA序列的分子方法带来了问题。本章描述了设计适用于分析近缘植物物种的PCR引物的方案。例如，我们对黑麦（Secale）的cpDNA非编码区域进行了PCR引物设计。