Morata de Ambrosini V I, Gonzalez S N, Oliver G
Centro de Referencia para Lactobacilos, Tucumán, Argentina.
J Food Prot. 1999 Dec;62(12):1430-4. doi: 10.4315/0362-028x-62.12.1430.
It is well known that the cell wall of Lactobacillus casei CRL 431, a strain present in probiotics, presents lectinlike surface molecules. Presence of these molecules stimulates the immune system. Given the role that lectins and lectinlike substances play in the adhesion phenomenon, it is probable that this is an initial stage in the immunostimulation produced by this bacterium. To confirm this, adhesion of this microorganism to exfoliated mouse ileal epithelial cells was studied in vitro. Other L. casei strains isolated from adult human intestines and one of dairy origin were also examined for their ability to adhere to ileal epithelial cells. Another strain, which was included in the present study, was Lactobacillus acidophilus CRL 730. L. casei strains isolated from humans showed good ability to adhere to ileal epithelial cells, whereas L. casei isolated from dairy origin did not. Adhesion was only observed at 37 degrees C and at a pH between 6 and 7.5. The exposure time needed for highest adhesion was 30 min. Presence of lectinlike substances on the surface of L. casei CRL 431 is important to this adhesion phenomenon, since adherence capacity was lost after removal of these substances.
众所周知，干酪乳杆菌CRL 431（一种存在于益生菌中的菌株）的细胞壁具有类凝集素表面分子。这些分子的存在会刺激免疫系统。鉴于凝集素和类凝集素物质在黏附现象中所起的作用，这很可能是该细菌产生免疫刺激的初始阶段。为了证实这一点，在体外研究了这种微生物与小鼠回肠脱落上皮细胞的黏附情况。还检测了从成人肠道分离出的其他干酪乳杆菌菌株以及一种源自乳制品的菌株黏附回肠上皮细胞的能力。本研究中纳入的另一个菌株是嗜酸乳杆菌CRL 730。从人类分离出的干酪乳杆菌菌株表现出良好的黏附回肠上皮细胞的能力，而从乳制品中分离出的干酪乳杆菌则没有这种能力。黏附仅在37摄氏度以及pH值在6至7.5之间时观察到。达到最高黏附所需的暴露时间为30分钟。干酪乳杆菌CRL 431表面类凝集素物质的存在对这种黏附现象很重要，因为去除这些物质后黏附能力丧失。
