Markowicz Corinna, Schmidt Marcin T
Department of Food Biotechnology and Microbiology, Poznań University of Life Sciences, Poland.
Acta Sci Pol Technol Aliment. 2015 Jul-Sep;14(3):247-255. doi: 10.17306/J.AFS.2015.3.26.
The ability of lactobacilli to adhere to the surface of the intestine is an important functional characteristic which can largely determine the effective colonization of the intestinal tract by probiotics. The following study compares the adhesion efficiency of the twenty strains of Lactobacillus genus belonging to Casei group to the Caco-2 cells and gastrointestinal mucus.
Twenty isolates of lactobacilli belonging to Casei group were tested. The ability of bacterial cells to adhere to mucus was examined using adhesion assay to gastrointestinal mucus. Obtained results were compared with adhesion efficiency to Caco-2 cells. Phylogenetic relationship between isolates was analysed by rep-PCR.
The results showed large differences in adhesion efficiency between strains, as well as differences in the efficiency of adhesion to the intestinal epithelial cells and mucus. Group similarity highlighted by a rep-PCR technique does not correspond with groups of similarity in terms of the characteristics of the ability to adhere to mucus or the epithelial cells of intestinal tract.
Strains having a high adhesion efficiency to enterocytes do not always show a high adhesion efficiency to the mucus. This may indicate the presence of different and multiple factors responsible for adhesion efficiency of Lactobacillus group Casei strains to epithelial cells and mucus.
乳酸菌黏附于肠道表面的能力是一项重要的功能特性，它在很大程度上能够决定益生菌在肠道中的有效定植。以下研究比较了干酪乳杆菌属20株属于干酪乳杆菌组的菌株对Caco-2细胞和胃肠道黏液的黏附效率。
对20株属于干酪乳杆菌组的乳酸菌分离株进行了测试。使用对胃肠道黏液的黏附试验检测细菌细胞黏附于黏液的能力。将获得的结果与对Caco-2细胞的黏附效率进行比较。通过rep-PCR分析分离株之间的系统发育关系。
结果显示菌株之间的黏附效率存在很大差异，以及对肠上皮细胞和黏液的黏附效率也存在差异。rep-PCR技术突出显示的组相似性与在黏附于黏液或肠道上皮细胞的能力特征方面的相似性组不对应。
对肠上皮细胞具有高黏附效率的菌株并不总是对黏液显示出高黏附效率。这可能表明存在不同的多种因素负责干酪乳杆菌组菌株对上皮细胞和黏液的黏附效率。
