双极膜电酸化法制备牛分离酪蛋白

Bipolar membrane electroacidification to produce bovine milk casein isolate.

作者信息

Bazinet L, Lamarche F, Ippersiel D, Amiot J

机构信息

Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Boulevard West, St. Hyacinthe, Québec, Canada J2S 8E3.

出版信息

J Agric Food Chem. 1999 Dec;47(12):5291-6. doi: 10.1021/jf990524m.

DOI:10.1021/jf990524m

PMID:10606611

Abstract

Bipolar membrane electroacidification (BMEA) has been developed previously (Bazinet et al., Report for the Canadian Electricity Association 9326 U 987, 1996; Bazinet et al., J. Agric. Food Chem. 1997, 45, 2419-2425, 3788-3794) and has been used for isoelectric precipitation of soybean proteins. The purpose of this study was to validate the feasibility of BMEA for the precipitation of milk casein and to investigate the effect of flow rate. High-purity isolates containing 1.23 and 2.00% ash and 85.4 and 91.6% total protein were obtained with flow rates of 0.2 and 1.2 gal/min. The molecular composition profiles of the isolates obtained by HPLC showed that only caseins were precipitated. However, except for protein precipitation curves, the flow rate did not influence the final composition and purity of the isolates. These results showed that BMEA is a new alternative process for the production of high-purity bovine milk casein isolate.

摘要

双极膜电酸化法（BMEA）此前已被开发出来（巴齐内特等人，加拿大电力协会报告9326 U 987，1996年；巴齐内特等人，《农业与食品化学杂志》，1997年，45卷，2419 - 2425页，3788 - 3794页），并已用于大豆蛋白的等电沉淀。本研究的目的是验证BMEA用于沉淀牛奶酪蛋白的可行性，并研究流速的影响。在流速为0.2和1.2加仑/分钟的情况下，获得了灰分含量分别为1.23%和2.00%、总蛋白含量分别为85.4%和91.6%的高纯度分离物。通过高效液相色谱法获得的分离物的分子组成图谱表明，只有酪蛋白被沉淀出来。然而，除了蛋白质沉淀曲线外，流速并不影响分离物的最终组成和纯度。这些结果表明，BMEA是生产高纯度牛乳酪蛋白分离物的一种新的替代方法。