Colón-Carmona A, You R, Haimovitch-Gal T, Doerner P
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Plant J. 1999 Nov;20(4):503-8. doi: 10.1046/j.1365-313x.1999.00620.x.
Plant growth responds rapidly to developmental and environmental signals, but the underlying changes in cell division activity are poorly understood. A labile cyclin-GUS reporter was developed to facilitate the spatio-temporal analysis of cell division patterns. The chimeric reporter protein is turned over every cell cycle and hence its histochemical activity accurately reports individual mitotic cells. Using Arabidopsis plants transformed with cyclin-GUS, we visualized patterns of mitotic activity in wounded leaves which suggest a role for cell division in structural reinforcement.
植物生长对发育和环境信号反应迅速，但细胞分裂活动背后的变化却知之甚少。为便于对细胞分裂模式进行时空分析，开发了一种不稳定的细胞周期蛋白 - GUS报告基因。嵌合报告蛋白在每个细胞周期都会更新，因此其组织化学活性能够准确报告单个有丝分裂细胞。利用转入细胞周期蛋白 - GUS的拟南芥植株，我们观察到受伤叶片中有丝分裂活动的模式，这表明细胞分裂在结构强化中发挥作用。
