Bryan D J, Wang K K, Summerhayes C
Department of Plastic and Reconstructive Surgery, R.E. Wise Research and Education Institute, Lahey Clinic, Burlington, Massachusetts 01805, USA.
J Reconstr Microsurg. 1999 Nov;15(8):591-6. doi: 10.1055/s-2007-1000143.
Schwann cells play a central role in peripheral-nerve regeneration, in which it has been shown that the addition of exogenous Schwann cells enhances the temporal and spatial sequence of events observed in regeneration. In this study, the authors investigated the fate of exogenous cells in this process by using fluorescently tagged autogenous Schwann cells in an established rat model of peripheral-nerve regeneration. Tracking labeled cells over a 4-week period revealed early migration of Schwann cells into the proximal nerve segment, followed by a concentration of migrating Schwann cells, leading the proximal growth cone throughout the regenerative process. The early proximal distribution of labeled cells suggests active migration in response to nerve damage, with spatial localization at the center of the proximal nerve segment and not the epineural surface. These observations demonstrate an interaction of exogenous Schwann cells with intact nerve tissue in vivo and affirm their role in the directional growth of regenerating axons.
施万细胞在周围神经再生中起着核心作用，研究表明，添加外源性施万细胞可增强再生过程中所观察到的事件的时间和空间顺序。在本研究中，作者通过在已建立的大鼠周围神经再生模型中使用荧光标记的自体施万细胞，研究了此过程中外源性细胞的命运。在4周的时间内追踪标记细胞，结果显示施万细胞早期迁移至近端神经段，随后迁移的施万细胞聚集，在整个再生过程中引导近端生长锥。标记细胞在近端的早期分布表明其对神经损伤做出了主动迁移反应，在近端神经段的中心而非神经外膜表面进行空间定位。这些观察结果证明了外源性施万细胞与体内完整神经组织之间的相互作用，并肯定了它们在再生轴突定向生长中的作用。
