ntrB和ntrC基因参与了巴西固氮螺菌Sp7中氨对聚-3-羟基丁酸生物合成的调控。
The ntrB and ntrC genes are involved in the regulation of poly-3-hydroxybutyrate biosynthesis by ammonia in Azospirillum brasilense Sp7.
作者信息
Sun J, Peng X, Van Impe J, Vanderleyden J
机构信息
F. A. Janssens Laboratory of Genetics, K.U. Leuven, B-3001 Heverlee, Belgium.
出版信息
Appl Environ Microbiol. 2000 Jan;66(1):113-7. doi: 10.1128/AEM.66.1.113-117.2000.
Abstract
Azospirillum brasilense Sp7 and its ntrA (rpoN), ntrBC, and ntrC mutants have been evaluated for their capabilities of poly-3-hydroxybutyrate (PHB) accumulation in media with high and low ammonia concentrations. It was observed that the ntrBC and ntrC mutants can produce PHB in both low- and high-C/N-ratio media, while no significant PHB production was observed for the wild type or the ntrA mutant in low-C/N-ratio media. Further investigation by fermentation analysis indicated that the ntrBC and ntrC mutants were able to grow and accumulate PHB simultaneously in the presence of a high concentration of ammonia in the medium, while little PHB was produced in the wild type and ntrA (rpoN) mutant during active growth phase. These results provide the first genetic evidence that the ntrB and ntrC genes are involved in the regulation of PHB synthesis by ammonia in A. brasilense Sp7.
摘要
巴西固氮螺菌Sp7及其ntrA（rpoN）、ntrBC和ntrC突变体已在高氨浓度和低氨浓度培养基中评估了其聚-3-羟基丁酸酯（PHB）积累能力。观察到ntrBC和ntrC突变体在低C/N比和高C/N比培养基中均能产生PHB，而在低C/N比培养基中未观察到野生型或ntrA突变体有显著的PHB产生。通过发酵分析进一步研究表明，ntrBC和ntrC突变体能够在培养基中存在高浓度氨的情况下同时生长并积累PHB，而野生型和ntrA（rpoN）突变体在活跃生长阶段几乎不产生PHB。这些结果提供了首个遗传学证据，表明ntrB和ntrC基因参与巴西固氮螺菌Sp7中氨对PHB合成的调控。
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