Rae AM, Howell EC, Kearsey MJ
Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham B15 2TT, U.K.
Heredity (Edinb). 1999 Nov;83 (Pt 5):586-96. doi: 10.1038/sj.hdy.6886050.
Seventy-nine recombinant backcross substitution lines from a cross between Brassica oleracea var. italica and Brassica oleracea var. alboglabra were grown in field trials over five years along with the alboglabra recurrent parent. Plants were scored for the days from sowing to the opening of the first flower, and lines that flowered significantly earlier or later than the recurrent parent were identified. Based on the lengths of the substitutions, evidence for 11 QTL on chromosomes O1, O2, O3, O5 and O9 was found, five of which mapped to similar regions to five of the six found in a previous analysis of doubled haploid lines from the same cross. Several of the QTL were linked closely in repulsion.
在五年的田间试验中,种植了79个源自意大利芥蓝(Brassica oleracea var. italica)和白花芥蓝(Brassica oleracea var. alboglabra)杂交的重组回交替换系,同时种植了白花芥蓝轮回亲本。记录了从播种到第一朵花开放的天数,并鉴定出开花时间明显早于或晚于轮回亲本的株系。根据替换片段的长度,在O1、O2、O3、O5和O9染色体上发现了11个QTL的证据,其中五个与之前对同一杂交的双单倍体系进行分析时发现的六个QTL中的五个定位到相似区域。几个QTL紧密连锁,呈相斥相。