Bohuon E J, Ramsay L D, Craft J A, Arthur A E, Marshall D F, Lydiate D J, Kearsey M J
Plant Genetics Group, School of Biological Sciences, The University of Birmingham, Birmingham B15 2TT, United Kingdom.
Genetics. 1998 Sep;150(1):393-401. doi: 10.1093/genetics/150.1.393.
A population of 150 doubled haploid lines of rapid cycling Brassica oleracea, derived from an F1 from a var. alboglabra x var. italica cross, was scored for flowering time in two trials. Using information on 82 mapped molecular markers, spread evenly across the nine linkage groups, QTL were identified at six locations; one each on linkage groups O2 and O3 and two each on linkage groups O5 and O9. In total, these QTL explained 58 and 93% of the genetical variation in the two trials. Three of these QTL, on linkage groups O2, O3, and O9, were situated in regions showing considerable homology both with each other and with chromosome regions of B. nigra that have been shown to affect flowering time. These same regions are all homologous to a single tract of Arabidopsis chromosome 5, which contains a number of the flowering-related genes, one or more of which may be candidates for the QTL found in Brassica.
从青花菜变种（var. alboglabra）和意大利芥蓝变种（var. italica）杂交产生的F1代中获得了150个快速循环的甘蓝型油菜双单倍体系，并在两项试验中对其开花时间进行了评分。利用82个均匀分布在9个连锁群上的已定位分子标记的信息，在6个位置鉴定出数量性状基因座（QTL）；连锁群O2和O3上各有1个，连锁群O5和O9上各有2个。总体而言，这些QTL在两项试验中分别解释了58%和93%的遗传变异。这些QTL中的3个，位于连锁群O2、O3和O9上，所在区域彼此之间以及与已被证明影响开花时间的黑芥染色体区域都显示出相当程度的同源性。这些相同区域都与拟南芥第5号染色体的一个单一区域同源，该区域包含许多开花相关基因，其中一个或多个可能是甘蓝型油菜中发现的QTL的候选基因。
