Ogborn M R, Nitschmann E, Weiler H A, Bankovic-Calic N
Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, Manitoba, Canada.
Kidney Int. 2000 Jan;57(1):159-66. doi: 10.1046/j.1523-1755.2000.00835.x.
Modification of polycystic kidney disease and fatty acid status by soy protein diet.
Previous studies have demonstrated that soy protein can slow progression of renal injury in the Han:SPRD-cy rat. We undertook a study to establish whether this benefit was independent of any nutritional deprivation, and whether or not it was associated with changes in polyunsaturated fatty acid status that have been previously linked to the anti-inflammatory or antineoplastic potential of soy diets.
Male Han:SPRD-cy rats were pair fed a 20% casein or 20% soy protein diet for six weeks from weaning. Tissue was harvested for analysis of cystic change, cell proliferation, macrophage infiltration, and fibrosis. Renal and hepatic tissues were also harvested for lipid analysis using gas chromatography.
Animals thrived on both diets. Soy protein feeding was associated with reduced cystic change (4.3 vs. 7.0 mL/kg, P < 0.0001), epithelial cell proliferation (15.7 vs. 21.0 cells/mm epithelium, P < 0.0001), macrophage infiltration (25.3 vs. 43.5 cells/high-power field, P < 0.0001), and fibrosis (0.6 vs. 1.07 mL/kg, P < 0.0001). The soy diet prevented a significant elevation in serum creatinine in diseased versus normal animals. Soy feeding was associated with higher renal and hepatic linoleic acid content and higher hepatic alpha-linolenic acid, but lower hepatic arachidonic acid content.
Isocaloric soy protein feeding ameliorates both epithelial and interstitial changes in the Han:SPRD-cy rat independent of a hypocholesterolemic effect. The histologic benefit is associated with changes in polyunsaturated fatty acid metabolism that may influence both inflammatory and proliferative pathways.
大豆蛋白饮食对多囊肾病和脂肪酸状态的影响
先前的研究表明，大豆蛋白可减缓Han:SPRD-cy大鼠肾损伤的进展。我们进行了一项研究，以确定这种益处是否独立于任何营养缺乏，以及它是否与多不饱和脂肪酸状态的变化有关，此前已将这些变化与大豆饮食的抗炎或抗肿瘤潜力联系起来。
雄性Han:SPRD-cy大鼠从断奶起成对喂食20%酪蛋白或20%大豆蛋白饮食六周。采集组织用于分析囊性变化、细胞增殖、巨噬细胞浸润和纤维化。还采集肾和肝组织用于气相色谱法脂质分析。
两种饮食喂养的动物均生长良好。喂食大豆蛋白与囊性变化减少（4.3 vs. 7.0 mL/kg，P < 0.0001）、上皮细胞增殖减少（15.7 vs. 21.0细胞/mm上皮，P < 0.0001）、巨噬细胞浸润减少（25.3 vs. 43.5细胞/高倍视野，P < 0.0001）和纤维化减少（0.6 vs. 1.07 mL/kg，P < 0.0001）相关。大豆饮食可防止患病动物与正常动物相比血清肌酐显著升高。喂食大豆与肾和肝亚油酸含量较高以及肝α-亚麻酸含量较高，但肝花生四烯酸含量较低相关。
等热量大豆蛋白喂养可改善Han:SPRD-cy大鼠的上皮和间质变化，与降胆固醇作用无关。组织学益处与多不饱和脂肪酸代谢的变化有关，这可能影响炎症和增殖途径。
