White P A, McIver C J, Deng Y, Rawlinson W D
Virology Division, Department of Microbiology, SEALS, Prince of Wales Hospital, Randwick, Sydney, N.S.W., Australia.
FEMS Microbiol Lett. 2000 Jan 15;182(2):265-9. doi: 10.1111/j.1574-6968.2000.tb08906.x.
Escherichia coli INS33 was isolated from the urinary tract of an infected patient. It was resistant to ampicillin, chloramphenicol, spectinomycin, streptomycin, sulfafurazole, tetracycline and trimethoprim. PCR screening revealed the presence of a class 1 integron that harboured two new gene cassettes, designated dfrA17 and aadA5. The new dfrA17 cassette was 91% identical to the known dfrA7 cassette. The aadA5 cassette was 95% identical over the first 830 bp to aadA4, but lacked the IS26 element found at the 3' end of this truncated cassette. Cloning and expression of the cassette region demonstrated that dfrA17 conferred high level resistance to trimethoprim but aadA5 conferred resistance to spectinomycin but not to streptomycin.
大肠杆菌INS33是从一名感染患者的尿道中分离出来的。它对氨苄青霉素、氯霉素、壮观霉素、链霉素、磺胺异恶唑、四环素和甲氧苄啶耐药。PCR筛选显示存在一个1类整合子，其中含有两个新的基因盒，分别命名为dfrA17和aadA5。新的dfrA17基因盒与已知的dfrA7基因盒有91%的同一性。aadA5基因盒在前830 bp与aadA4有95%的同一性，但缺少在这个截短基因盒3'端发现的IS26元件。基因盒区域的克隆和表达表明，dfrA17赋予对甲氧苄啶的高水平耐药性，而aadA5赋予对壮观霉素的耐药性，但对链霉素不耐药。
