Reid B J, Lee P H, Macleod C J, Morriss A W, Patel D, Semple K T
Department of Environmental Science, Institute of Environmental and Natural Sciences, Lancaster University, Lancaster, UK.
FEMS Microbiol Lett. 2000 Jan 15;182(2):367-73. doi: 10.1111/j.1574-6968.2000.tb08923.x.
Subsurface high voltage electric cables are commonly insulated using dodecylbenzene in combination with mineral oil. This work assessed the impact of increasing concentrations of cable insulating oil (0-10% dry weight) on soil microbial respiration as determined by mineralisation of [1-(14)C]glucose (11 microg C g(-1) soil). Acute impact was assessed from 0 days to 21 days, and chronic impact was assessed after 300 days. This study found that cable insulating oil increased respiratory activity of soil microflora. The extent of impact was found to depend on both oil concentration and the length of oil-soil contact time. Following acute exposure (21-days oil-soil contact time), it was found that oil concentrations up to 1% promoted a significant (P<0.05) increase in the extent of [1-(14)C]glucose mineralisation to (14)CO(2) relative to the control. In contrast, higher concentrations of cable insulating oil (5% and 10%) promoted no significant (P0.05) increase in the extent of [1-(14)C]glucose mineralisation to (14)CO(2) relative to the control. Following chronic exposure (300-days oil-soil contact time), the extent of mineralisation was greater at all oil concentrations applied relative to the control. For oil concentrations up to and including 1%, there was a decrease in the extent of elevation in mineralisation relative to the values after 21-days exposure. At higher oil concentrations, namely 5% and 10%, the extent of elevation in mineralisation was comparable with that after 21-days oil-soil contact time. We suggest that the increase in mineralisation of glucose indicates that cable insulating oil is a readily available carbon source to the carbon-limited soil microflora.
地下高压电缆通常使用十二烷基苯与矿物油混合进行绝缘。这项工作评估了电缆绝缘油浓度增加（0 - 10%干重）对土壤微生物呼吸的影响，该影响通过[1 - (14)C]葡萄糖（11微克碳/克土壤）的矿化作用来测定。从0天到21天评估急性影响，300天后评估慢性影响。本研究发现电缆绝缘油增加了土壤微生物群落的呼吸活性。发现影响程度取决于油浓度和油与土壤接触时间的长短。急性暴露（油与土壤接触21天）后，发现油浓度高达1%时，相对于对照，[1 - (14)C]葡萄糖矿化生成(14)CO(2)的程度显著（P<0.05）增加。相比之下，较高浓度的电缆绝缘油（5%和10%）相对于对照，[1 - (14)C]葡萄糖矿化生成(14)CO(2)的程度没有显著（P>0.05）增加。慢性暴露（油与土壤接触300天）后，相对于对照，所有施加的油浓度下矿化程度都更高。对于油浓度高达并包括1%的情况，相对于21天暴露后的数值，矿化增加程度有所下降。在较高油浓度即5%和10%时，矿化增加程度与油与土壤接触21天后相当。我们认为葡萄糖矿化的增加表明电缆绝缘油是碳受限土壤微生物群落易于利用的碳源。
