Fouchard N, Lacoste V, Couppie P, Develoux M, Mauclere P, Michel P, Herve V, Pradinaud R, Bestetti G, Huerre M, Tekaia F, de Thé G, Gessain A
Unité d'Epidémiologie des Virus Oncogènes, Département SIDA et Rétrovirus, Institut Pasteur, Paris, France.
Int J Cancer. 2000 Jan 15;85(2):166-70.
Kaposi's-sarcoma-associated herpesvirus(KSHV)/human-herpes-virus-8(HHV-8) sequences originally detected in AIDS-associated Kaposi's sarcoma have been found in almost every KS tested, whether endemic, classic, iatrogenic or epidemic. Most of the studies on African KS involved East African patients. We report herewith the study of 17 African or Guyanan KS patients, 3 with epidemic KS (EKS) from Central African Republic, 3 from Senegal (2 EKS and 1 endemic KS), 3 EKS from Cameroon and 8 from French Guiana (3 EKS and 5 endemic KS). Serum-specific antibodies directed against latent and/or lytic HHV-8 antigens were present in 16 of them (94%), detected either by immunofluorescence assay and/or by immunoperoxidase. Polymerase chain reaction (PCR), using specific primers for HHV-8 ORF26 (233 bp) and ORF75 (601 bp), was carried out on DNA extracted from KS cutaneous biopsies, clinically uninvolved skin biopsies and peripheral-blood mononuclear cells (PBMC). HHV-8 DNA was detected in 16 out of 16 (100%) KS biopsies, regardless of their origin or clinico-pathological sub-type, in 7 out of 15 (47%) normal skin samples and 7 out of 16 (44%) PBMC. Comparative PCR, carried out in 7 patients, regularly found a much higher viral load in KS biopsies than in autologous normal skin and PBMC samples. Sequencing of fragments of the ORF26 and of the ORF75 demonstrated that the 16 HHV-8 strains were of the A, B or C sub-type. Furthermore, sequences of the entire ORF K1 of 4 strains showed that these HHV-8 strains of African origin were of the A5 or the B sub-type.
最初在与艾滋病相关的卡波西肉瘤中检测到的卡波西肉瘤相关疱疹病毒（KSHV）/人类疱疹病毒8型（HHV-8）序列，在几乎所有检测的卡波西肉瘤中都能找到，无论其为地方性、经典型、医源性还是流行性。大多数关于非洲卡波西肉瘤的研究涉及东非患者。我们在此报告对17例非洲或圭亚那卡波西肉瘤患者的研究，其中3例来自中非共和国的流行性卡波西肉瘤（EKS）患者、3例来自塞内加尔（2例EKS和1例地方性卡波西肉瘤）、3例来自喀麦隆的EKS患者以及8例来自法属圭亚那（3例EKS和5例地方性卡波西肉瘤）。其中16例（94%）患者体内存在针对潜伏性和/或裂解性HHV-8抗原的血清特异性抗体，通过免疫荧光法和/或免疫过氧化物酶法检测到。使用针对HHV-8 ORF26（233 bp）和ORF75（601 bp）的特异性引物进行聚合酶链反应（PCR），对从卡波西肉瘤皮肤活检组织、临床未受累皮肤活检组织以及外周血单核细胞（PBMC）中提取的DNA进行检测。在16例（100%）卡波西肉瘤活检组织中均检测到HHV-8 DNA，无论其来源或临床病理亚型如何；在15例正常皮肤样本中的7例（47%）以及16例PBMC中的7例（44%）检测到HHV-8 DNA。对7例患者进行的比较PCR结果显示，卡波西肉瘤活检组织中的病毒载量通常远高于自体正常皮肤和PBMC样本。对ORF26和ORF75片段进行测序表明，这16株HHV-8毒株属于A、B或C亚型。此外，对4株毒株的整个ORF K1序列分析表明，这些源自非洲的HHV-8毒株属于A5或B亚型。
