鼠伤寒沙门氏菌σ(54)的氨基末端是与增强子结合蛋白相互作用及与叉状连接DNA结合所必需的。
The amino terminus of Salmonella enterica serovar Typhimurium sigma(54) is required for interactions with an enhancer-binding protein and binding to fork junction DNA.
作者信息
Kelly M T, Hoover T R
机构信息
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
出版信息
J Bacteriol. 2000 Jan;182(2):513-7. doi: 10.1128/JB.182.2.513-517.2000.
Abstract
Transcription initiation by the sigma(54)-RNA polymerase holoenzyme requires an enhancer-binding protein that is thought to contact sigma(54) to activate transcription. To identify potential enhancer-binding protein contact sites in sigma(54), we compared the abilities of wild-type and truncated forms of Salmonella enterica serovar Typhimurium sigma(54) to interact with the enhancer-binding protein DctD in a chemical cross-linking assay. Removal of two regions in the amino-terminal portion of sigma(54), residues 57 to 105 and residues 144 to 179, prevented cross-linking, but removal of either region alone did not. In addition, deletion of 56 amino-terminal residues of sigma(54) (region I) reduced the affinity of the protein for a fork junction DNA probe.
摘要
由σ⁵⁴ -RNA聚合酶全酶引发的转录起始需要一种增强子结合蛋白，该蛋白被认为通过与σ⁵⁴接触来激活转录。为了鉴定σ⁵⁴中潜在的增强子结合蛋白接触位点，我们在化学交联实验中比较了鼠伤寒沙门氏菌σ⁵⁴的野生型和截短形式与增强子结合蛋白DctD相互作用的能力。去除σ⁵⁴氨基末端部分的两个区域，即第57至105位残基和第144至179位残基，会阻止交联，但单独去除任何一个区域则不会。此外，删除σ⁵⁴的56个氨基末端残基（区域I）会降低该蛋白对叉形连接DNA探针的亲和力。
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本文引用的文献
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Sequences in sigmaN determining holoenzyme formation and properties.σN中决定全酶形成和性质的序列。
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