新型荧光CDP类似物（Pβ）MABA - CDP是UMP/CMP激酶NMP结合位点的特异性探针。
The novel fluorescent CDP-analogue (Pbeta)MABA-CDP is a specific probe for the NMP binding site of UMP/CMP kinase.
作者信息
Rudolph M G, Veit T J, Reinstein J
机构信息
Max-Planck-Institut für Molekulare Physiologie, Abetilung Physikalische Biochemie, Dormund, Germany.
出版信息
Protein Sci. 1999 Dec;8(12):2697-704. doi: 10.1110/ps.8.12.2697.
Abstract
Direct thermodynamic and kinetic investigations of the binding of nucleotides to the nucleoside monophosphate (NMP) site of NMP kinases have not been possible so far because a spectroscopic probe was not available. By coupling a fluorescent N-methylanthraniloyl- (mant) group to the beta-phosphate of CDP via a butyl linker, a CDP analogue [(Pbeta)MABA-CDP] was obtained that still binds specifically to the NMP site of UmpKdicty, because the base and the ribose moieties, which are involved in specific interactions, are not modified. This allows the direct determination of binding constants for its substrates in competition experiments.
摘要
到目前为止，由于缺乏合适的光谱探针，无法对核苷酸与NMP激酶的核苷单磷酸（NMP）位点的结合进行直接的热力学和动力学研究。通过经由丁基连接子将荧光N-甲基邻氨基苯甲酰基-（mant）基团连接到CDP的β-磷酸基团上，获得了一种CDP类似物[（Pβ）MABA-CDP]，它仍然能特异性地结合到UmpKdicty的NMP位点，因为参与特异性相互作用的碱基和核糖部分未被修饰。这使得在竞争实验中能够直接测定其底物的结合常数。
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本文引用的文献
1
On the attribution and additivity of binding energies.关于结合能的归因和可加性。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4046-50. doi: 10.1073/pnas.78.7.4046.
2
GTP analogue hydrolysis by the Gs protein: implication for the role of catalytic glutamine in the GTPase reaction.Gs蛋白对GTP类似物的水解作用：催化性谷氨酰胺在GTP酶反应中的作用探讨
FEBS Lett. 1998 Aug 21;433(3):326-30. doi: 10.1016/s0014-5793(98)00930-2.
3
Crystal structure of yeast thymidylate kinase complexed with the bisubstrate inhibitor P1-(5'-adenosyl) P5-(5'-thymidyl) pentaphosphate (TP5A) at 2.0 A resolution: implications for catalysis and AZT activation.酵母胸苷酸激酶与双底物抑制剂P1-(5'-腺苷基)P5-(5'-胸苷基)五磷酸(TP5A)复合物在2.0埃分辨率下的晶体结构：对催化作用和齐多夫定激活的影响
Biochemistry. 1998 Mar 17;37(11):3677-86. doi: 10.1021/bi9720787.
4
Rescue of a mutant G-protein by substrate-assisted catalysis.通过底物辅助催化挽救突变型G蛋白。
Eur J Biochem. 1997 Oct 1;249(1):330-6. doi: 10.1111/j.1432-1033.1997.00330.x.
5
The bottleneck in AZT activation.齐多夫定激活过程中的瓶颈
Nat Med. 1997 Aug;3(8):922-4. doi: 10.1038/nm0897-922.
6
The second step of ATP binding to DnaK induces peptide release.ATP与DnaK结合的第二步会诱导肽段释放。
J Mol Biol. 1996 Nov 15;263(5):657-70. doi: 10.1006/jmbi.1996.0606.
7
Sensing the heat: the application of isothermal titration calorimetry to thermodynamic studies of biomolecular interactions.感知热量：等温滴定量热法在生物分子相互作用热力学研究中的应用。
Chem Biol. 1996 Oct;3(10):791-801. doi: 10.1016/s1074-5521(96)90063-0.
8
Analytical properties of the nanoelectrospray ion source.纳米电喷雾离子源的分析特性。
Anal Chem. 1996 Jan 1;68(1):1-8. doi: 10.1021/ac9509519.
9
Crystal structure of the complex of UMP/CMP kinase from Dictyostelium discoideum and the bisubstrate inhibitor P1-(5'-adenosyl) P5-(5'-uridyl) pentaphosphate (UP5A) and Mg2+ at 2.2 A: implications for water-mediated specificity.盘基网柄菌UMP/CMP激酶与双底物抑制剂P1-(5'-腺苷基)P5-(5'-尿苷基)五磷酸(UP5A)及Mg2+复合物在2.2埃分辨率下的晶体结构：对水介导特异性的影响
Biochemistry. 1996 Jul 30;35(30):9716-27. doi: 10.1021/bi960642s.
10
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