致力于开发一种用于布氏冈比亚锥虫治疗随访的诊断方案。
Towards developing a diagnostic regimen for the treatment follow-up of Trypanosoma brucei gambiense.
作者信息
Mbati P A, Hirumi K, Inoue N, Situakibanza N H, Hirumi H
机构信息
University of the North, Parasitology Research Program, Phuthaditjaba, South Africa.
出版信息
Korean J Parasitol. 1999 Dec;37(4):289-92. doi: 10.3347/kjp.1999.37.4.289.
BALB/c mice infected with a high virulent strain of Trypanosoma brucei gambiense IL3707 were treated intraperitoneally (i.p.) with either Melarsoprol (Mel-B) or PSG(+) buffer as controls. The mice were subsequently monitored regularly for parasites by direct microscopic examination of their tail blood or buffy coat and by polymerase chain reaction (PCR). Mel-B was found to be an effective drug for treatment against T.b. gambiense because at the end of the first treatment schedule, all treated mice were negative for parasites even by PCR, while all the control animals were positive. Three of the five Mel-B treated mice, while parasitologically negative, were PCR positive between 53 and 80 days post infection (DPI), indicating that they still harbored an infection. All treated mice were subsequently negative for parasites even by PCR at 88 DPI. A combination of conventional microscopic examination and PCR offers a good prediction of cure following treatment of trypanosomosis.
用高毒力冈比亚布氏锥虫IL3707株感染的BALB/c小鼠，腹腔注射美拉胂醇（Mel-B）或PSG(+)缓冲液作为对照进行治疗。随后通过直接显微镜检查小鼠尾血或血沉棕黄层以及聚合酶链反应（PCR）定期监测小鼠体内的寄生虫。发现Mel-B是治疗冈比亚布氏锥虫的有效药物，因为在第一个治疗疗程结束时，所有接受治疗的小鼠即使通过PCR检测寄生虫也呈阴性，而所有对照动物均为阳性。五只接受Mel-B治疗的小鼠中有三只，虽然寄生虫学检测呈阴性，但在感染后53至80天之间PCR检测呈阳性，这表明它们仍有感染。在感染后88天，所有接受治疗的小鼠即使通过PCR检测寄生虫也呈阴性。传统显微镜检查和PCR相结合能很好地预测锥虫病治疗后的治愈情况。
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