Inoue N, Narumi D, Mbati P A, Hirumi K, Situakibanza N T, Hirumi H
The Research Centre for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, Japan.
Trop Med Int Health. 1998 May;3(5):408-12. doi: 10.1046/j.1365-3156.1998.00245.x.
Susceptibility of severe combined immunodeficient (SCID) mice to 7 isolates of Trypanosoma brucei gambiense and 2 isolates of T. b. rhodesiense was examined in terms of their infectivity, course of parasitaemia, packed cell volume (PCV) and survival period in comparison with that of normal immunocompetent (BALB/c) mice. All isolates of T. b. gambiense and T. b. rhodesiense caused high (> 1 x 10(8) parasites/ml) parasitaemia in the SCID mice, the survival periods ranged from 5 to 47 days. On the other hand, 5 of 7 isolates of T. b. gambiense developed chronic infection in the BALB/c mice with sporadic but persistent parasitaemia with less than 5 x 10(6) parasites/ml. All the mice tested in this group survived more than 60 days after infection. In contrast, the 2 remaining isolates of T. b. gambiense and both isolates of T. b. rhodesiense showed high virulence in the BALB/c mice and killed all of them within 30 days after infection. The results demonstrate that the SCID mice, in which functional B- and T-cell-mediated immunities are congenitally lacking, are highly susceptible for 'low-virulence' T. b. gambiense. This makes SCID mice useful tools for the isolation of parasites from T. b. gambiense sleeping sickness patients and the propagation of large amounts of such parasites.
研究了严重联合免疫缺陷（SCID）小鼠对7株布氏冈比亚锥虫和2株布氏罗得西亚锥虫的易感性，包括它们的感染性、寄生虫血症过程、血细胞比容（PCV）和生存期，并与正常免疫功能（BALB/c）小鼠进行了比较。所有布氏冈比亚锥虫和布氏罗得西亚锥虫分离株在SCID小鼠中均引起高寄生虫血症（>1×10⁸个寄生虫/ml），生存期为5至47天。另一方面，7株布氏冈比亚锥虫分离株中的5株在BALB/c小鼠中引发慢性感染，寄生虫血症呈散发性但持续存在，寄生虫数量少于5×10⁶个/ml。该组中所有受试小鼠在感染后存活超过60天。相比之下，其余2株布氏冈比亚锥虫分离株和2株布氏罗得西亚锥虫分离株在BALB/c小鼠中显示出高毒力，在感染后30天内将所有小鼠致死。结果表明，先天性缺乏功能性B细胞和T细胞介导免疫的SCID小鼠对“低毒力”的布氏冈比亚锥虫高度易感。这使得SCID小鼠成为从布氏冈比亚锥虫昏睡病患者中分离寄生虫以及大量繁殖此类寄生虫的有用工具。
