Hallet B, Arciszewska L K, Sherratt D J
Department of Biochemistry, University of Oxford, United Kingdom.
Mol Cell. 1999 Dec;4(6):949-59. doi: 10.1016/s1097-2765(00)80224-5.
In Xer site-specific recombination, sequential DNA strand exchange reactions are catalyzed by a heterotetrameric complex composed of two recombinases, XerC and XerD. It is demonstrated that XerC and XerD catalytic activity is controlled by an interaction involving the C-terminal end of each protein (the donor region) and an internal region close to the active site (the acceptor region). Mutations in these regions reciprocally alter the relative activity of XerC and XerD, with their combination producing synergistic effects on catalysis. The data support a model in which C-terminal intersubunit interactions contribute to coupled protein-DNA conformational changes that lead to sequential activation and reciprocal inhibition of pairs of active sites in the recombinase tetramer during recombination.
在Xer位点特异性重组中，由两种重组酶XerC和XerD组成的异源四聚体复合物催化连续的DNA链交换反应。已证明，XerC和XerD的催化活性受一种相互作用的控制，该相互作用涉及每种蛋白质的C末端（供体区域）和靠近活性位点的内部区域（受体区域）。这些区域的突变相互改变XerC和XerD的相对活性，它们的组合对催化产生协同效应。这些数据支持一种模型，即C末端亚基间相互作用有助于蛋白质-DNA构象的耦合变化，从而在重组过程中导致重组酶四聚体中活性位点对的顺序激活和相互抑制。
