携带有 qnrB19 的小质粒：一个通过 oriT 和 Xer 位点的位点特异性重组介导的质粒进化模型。
Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites.
机构信息
Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, California, USA.
出版信息
Antimicrob Agents Chemother. 2012 Apr;56(4):1821-7. doi: 10.1128/AAC.06036-11. Epub 2012 Jan 30.
Abstract
Plasmids pPAB19-1, pPAB19-2, pPAB19-3, and pPAB19-4, isolated from Salmonella and Escherichia coli clinical strains from hospitals in Argentina, were completely sequenced. These plasmids include the qnrB19 gene and are 2,699, 3,082, 2,989, and 2,702 nucleotides long, respectively, and they share extensive homology among themselves and with other previously described small qnrB19-harboring plasmids. The genetic environment of qnrB19 in all four plasmids is identical to that in these other plasmids and in transposons such as Tn2012, Tn5387, and Tn5387-like. Nucleotide sequence comparisons among these and previously described plasmids showed a variable region characterized by being flanked by an oriT locus and a Xer recombination site. We propose that this arrangement could play a role in the evolution of plasmids and present a model for DNA swapping between plasmid molecules mediated by site-specific recombination events at oriT and a Xer target site.
摘要
从阿根廷医院的沙门氏菌和大肠杆菌临床分离株中分离得到的质粒 pPAB19-1、pPAB19-2、pPAB19-3 和 pPAB19-4 已被完全测序。这些质粒均包含 qnrB19 基因，长度分别为 2699、3082、2989 和 2702 个核苷酸，它们彼此之间以及与其他先前描述的携带小 qnrB19 的质粒具有广泛的同源性。qnrB19 在所有四个质粒中的遗传环境与其他质粒以及转座子（如 Tn2012、Tn5387 和 Tn5387 样）中的环境相同。对这些质粒和先前描述的质粒之间的核苷酸序列比较表明，存在一个由 oriT 位点和 Xer 重组位点侧翼的可变区域。我们提出，这种排列可能在质粒的进化中发挥作用，并提出了一个模型，用于在 oriT 和 Xer 靶位点的位点特异性重组事件介导下，质粒分子之间的 DNA 交换。
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