使用白细胞游走抑制试验证明慢性粒细胞白血病中的细胞免疫。
Demonstration of cellular immunity in chronic myeloid leukaemia using leucocyte migration inhibition assay.
作者信息
Gangal S G, Gothoskar B P, Joshi C S, Advani S H
出版信息
Br J Cancer. 1976 Mar;33(3):267-72. doi: 10.1038/bjc.1976.39.
Peripheral blood leucocytes from chronic myeloid leukaemia patients in remission were tested for inhibition of migration in presence of solubilized membrane antigens from leukaemic cells in 15 cases. Eight out of 9 autochthonous combinations (88-8%) and 35/49 allogenic combinations (71-4%) showed inhibition of migration. Antigens prepared from relapse leukaemic cell samples in 4 cases showed inhibition of migration of autochthonous as well as allogeneic remission leucocytes. The same batch of CML antigens inhibited migration of normal leucocytes at the level of 22-2%. The difference between inhibition of migration shown by remission leucocytes and normal leucocytes in presence of CML antigens was statistically significant. Solubilized antigens, similarly prepared from normal leucocytes, showed inhibition of migration of remission leucocytes to the extent of 15% only. The difference between the reactivity of CML remission leucocytes to normal and CML antigens was also statistically significant. No enhancement of migration of remission leucocytes was seen with CML antigens.
对15例慢性粒细胞白血病缓解期患者的外周血白细胞进行检测，观察其在白血病细胞溶解膜抗原存在情况下的迁移抑制情况。9例自身组合中有8例（88.8%）以及49例异体组合中有35例（71.4%）出现迁移抑制。4例复发白血病细胞样本制备的抗原对自身及异体缓解期白细胞的迁移均有抑制作用。同一批慢性粒细胞白血病抗原对正常白细胞迁移的抑制率为22.2%。在慢性粒细胞白血病抗原存在情况下，缓解期白细胞与正常白细胞迁移抑制情况的差异具有统计学意义。同样由正常白细胞制备的溶解抗原，对缓解期白细胞迁移的抑制率仅为15%。慢性粒细胞白血病缓解期白细胞对正常抗原与慢性粒细胞白血病抗原反应性的差异也具有统计学意义。慢性粒细胞白血病抗原未使缓解期白细胞迁移增强。
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