Somasundaram R, Advani S H, Gangal S G
Br J Cancer. 1983 Dec;48(6):783-90. doi: 10.1038/bjc.1983.267.
In the present paper attempts have been made to investigate suppressor cell activity in CML patients in first and subsequent remissions in order to study the relationship between suppressor cell activity and progression of the disease. For this purpose, the ability of Con A activated suppressor cells from peripheral blood of CML patients in 1st, 2nd and 3rd remission to suppress PHA response of autologous lymphocytes is investigated and compared with that of normal healthy donors. The ability of Con A activated cell population to form rosettes with autologous RBCs (ARFC) is also investigated. The results indicate that lymphocytes from CML patients in 1st (61.8 +/- 6.1%), 2nd (62.6 +/- 3.0%) and 3rd (55.3 +/- 4.8%) remissions show significantly high suppressor cell activity than normal healthy donors (36.5 +/- 1.9%) when activated with Con A. Similarly, generation of spontaneous suppressor cell activity was also higher in 1st (23.3 +/- 4.7%) and 2nd (25.3 +/- 4.2%) remission lymphocytes than controls (10.1 +/- 2.5%). In the 3rd remission however, the spontaneous suppressor cell activity (14.5 +/- 3.2%) was comparable to controls. Thus it appears that a higher suppressor cell precursor population is present in CML patients in remission. However, this could not be correlated with the progression of the disease. CML patients in 1st remission also revealed an increased percentage of ARFC which correlated with the suppressor cell function. The ARFC activity tested in a few patients in subsequent remissions was comparable with controls although functional suppressor activity was increased.
在本论文中，已尝试研究慢性粒细胞白血病（CML）患者首次缓解期及后续缓解期的抑制细胞活性，以探讨抑制细胞活性与疾病进展之间的关系。为此，研究了处于第1次、第2次和第3次缓解期的CML患者外周血中经刀豆蛋白A（Con A）激活的抑制细胞抑制自身淋巴细胞对PHA反应的能力，并与正常健康供体进行比较。还研究了经Con A激活的细胞群体与自身红细胞形成花环（ARFC）的能力。结果表明，处于第1次（61.8±6.1%）、第2次（62.6±3.0%）和第3次（55.3±4.8%）缓解期的CML患者的淋巴细胞在用Con A激活后，其抑制细胞活性显著高于正常健康供体（36.5±1.9%）。同样，第1次（23.3±4.7%）和第2次（25.3±4.2%）缓解期淋巴细胞的自发抑制细胞活性也高于对照组（10.1±2.5%）。然而，在第3次缓解期，自发抑制细胞活性（14.5±3.2%）与对照组相当。因此，似乎缓解期的CML患者中存在较高的抑制细胞前体细胞群体。然而，这与疾病的进展并无关联。处于第1次缓解期的CML患者还显示ARFC百分比增加，这与抑制细胞功能相关。在后续缓解期的少数患者中测试的ARFC活性与对照组相当，尽管功能性抑制活性有所增加。
