使用连接酶链反应分析法评估女性自行采集的样本中是否存在沙眼衣原体生殖器感染。

Evaluation of self-taken samples for the presence of genital Chlamydia trachomatis infection in women using the ligase chain reaction assay.

作者信息

Carder C, Robinson A J, Broughton C, Stephenson J M, Ridgway G L

机构信息

Department of Clinical Microbiology, University College London Hospitals, UK.

出版信息

Int J STD AIDS. 1999 Dec;10(12):776-9. doi: 10.1258/0956462991913538.

DOI:10.1258/0956462991913538

PMID:10639056

Abstract

The aim of this study was to evaluate the sensitivity and acceptability of self-taken vulval-introital (VI) samples, first-catch urine (FCU) samples and clinician-obtained cervical samples for the presence of genital Chlamydia trachomatis infections in women using the ligase chain reaction (LCR) assay. One hundred and four patients were enrolled, of whom 54 patients had chlamydial DNA in at least one of the samples tested. The sensitivity of the cervical sample was 96.3%, vulval-introital sample in LCR buffer 92.6%, vulval-introital swab collected dry 88.9%, FCU stored at +2-8 degrees C 81.5%, FCU stored at room temperature 77.8% and FCU stored with 2% w/v boric acid at room temperature 87.0%. Self-taken vulval-introital LCR samples were shown to be an acceptable alternative to a clinician-obtained LCR sample. The addition of boric acid may overcome the need for a continuous cold chain for FCU samples.

摘要

本研究旨在使用连接酶链反应（LCR）检测法，评估自行采集的外阴-阴道（VI）样本、首次晨尿（FCU）样本以及临床医生采集的宫颈样本对于检测女性生殖器沙眼衣原体感染的敏感性和可接受性。共招募了104名患者，其中54名患者至少在一种检测样本中检测到衣原体DNA。宫颈样本的敏感性为96.3%，LCR缓冲液中的外阴-阴道样本为92.6%，干燥采集的外阴-阴道拭子为88.9%，2-8摄氏度储存的FCU为81.5%，室温储存的FCU为77.8%，室温下添加2%（w/v）硼酸储存的FCU为87.0%。自行采集的外阴-阴道LCR样本被证明是临床医生采集的LCR样本的可接受替代方法。添加硼酸可能无需对FCU样本进行连续冷链保存。