幽门螺杆菌免疫显性抗原的鉴定及其与不同胃十二指肠病变患者血清反应性的评估。
Identification of immunodominant antigens from Helicobacter pylori and evaluation of their reactivities with sera from patients with different gastroduodenal pathologies.
作者信息
Kimmel B, Bosserhoff A, Frank R, Gross R, Goebel W, Beier D
机构信息
Theodor-Boveri-Institut für Biowissenschaften, Lehrstuhl für Mikrobiologie, Universität Würzburg, D-97074 Würzburg, Germany.
出版信息
Infect Immun. 2000 Feb;68(2):915-20. doi: 10.1128/IAI.68.2.915-920.2000.
Abstract
Colonization of the gastric mucosa by Helicobacter pylori is the major cause of gastroduodenal pathologies in humans. Studying the outcome of the humoral immune response directed against this gastric pathogen may contribute substantially to vaccine development and to the improvement of diagnostic techniques based on serology. By using two-dimensional gel electrophoresis, 29 proteins from H. pylori G27 were identified which strongly react with sera derived from H. pylori-infected patients suffering from different gastroduodenal pathologies. These antigens were characterized by mass spectrometry and proved to correspond to products of open reading frames predicted by the H. pylori genome sequence. The comparison of the antigenic patterns recognized by these sera revealed no association of specific H. pylori antigens with antibodies in patients with particular gastroduodenal pathologies.
摘要
幽门螺杆菌在胃黏膜的定植是人类胃十二指肠疾病的主要病因。研究针对这种胃部病原体的体液免疫反应结果，可能会对疫苗研发以及基于血清学的诊断技术改进做出重大贡献。通过二维凝胶电泳，鉴定出幽门螺杆菌G27的29种蛋白质，它们与来自患有不同胃十二指肠疾病的幽门螺杆菌感染患者的血清发生强烈反应。这些抗原通过质谱进行了表征，并证明与幽门螺杆菌基因组序列预测的开放阅读框产物相对应。对这些血清识别的抗原模式进行比较发现，特定的幽门螺杆菌抗原与患有特定胃十二指肠疾病患者体内的抗体之间没有关联。
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