用弓形虫SAG1进行鼻内免疫可诱导保护性细胞进入鼻相关淋巴组织（NALT）和肠相关淋巴组织（GALT）区室。
Intranasal immunization with Toxoplasma gondii SAG1 induces protective cells into both NALT and GALT compartments.
作者信息
Velge-Roussel F, Marcelo P, Lepage A C, Buzoni-Gatel D, Bout D T
机构信息
Equipe Associée INRA d'Immunologie Parasitaire-EA 2637, UFR des Sciences Pharmaceutiques, 37200 Tours, France.
出版信息
Infect Immun. 2000 Feb;68(2):969-72. doi: 10.1128/IAI.68.2.969-972.2000.
Abstract
Intranasal (i.n.) immunization with the SAG1 protein of Toxoplasma gondii plus cholera toxin (CT) provides protective immunity. The aim of this study was to analyze the cellular activation of several mucosal compartments after i.n. immunization. Cervical and mesenteric lymph node (CLN and MLN, respectively) lymphoid cell and intraepithelial lymphocyte (IEL) passive transfer experiments were performed with CBA/J mice immunized i.n. with SAG1 plus CT. CLN and MLN cells and IEL isolated 42 days after immunization conferred protective immunity on naive recipient mice challenged with strain 76K T. gondii, as assessed by the reduction in the number of brain cysts. There were proliferative specific responses in nose-associated lymphoid tissue and the CLN and MLN cells from mice immunized with SAG1 plus CT, but no cytokine was detectable. Thus, protective immunity is associated with a specific cellular response in the nasal and mesenteric compartments after i.n. immunization.
摘要
用刚地弓形虫的SAG1蛋白加霍乱毒素（CT）进行鼻内（i.n.）免疫可提供保护性免疫。本研究的目的是分析鼻内免疫后几个黏膜区室的细胞活化情况。对用SAG1加CT进行鼻内免疫的CBA/J小鼠进行了颈淋巴结和肠系膜淋巴结（分别为CLN和MLN）淋巴细胞及上皮内淋巴细胞（IEL）的被动转移实验。免疫后42天分离的CLN和MLN细胞以及IEL赋予了对用76K刚地弓形虫株攻击的未免疫受体小鼠的保护性免疫，这通过脑囊肿数量的减少来评估。在用SAG1加CT免疫的小鼠的鼻相关淋巴组织以及CLN和MLN细胞中存在增殖性特异性反应，但未检测到细胞因子。因此，保护性免疫与鼻内免疫后鼻和肠系膜区室中的特异性细胞反应相关。
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